BMRB: 34276

Tc-DNA/RNA duplex

Authors

Istrate, A., Johannsen, S., Istrate, A., Sigel, R.K.O., Leumann, C.

Assembly

Tc-DNA/RNA duplex

Entity

1. Tc-DNA/RNA duplex, entity 1 (polymer, Thiol state: not present), 10 monomers, 3495.526 Da
2. Tc-DNA/RNA duplex, entity 2 (polymer, Thiol state: not present), 10 monomers, 3302.984 Da Detail

GUAAGCCGAG

Total weight

6798.51 Da

Max. entity weight

3495.526 Da

Source organism

synthetic construct , Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 50.0 %, Completeness: 94.0 %, Completeness (bb): 93.6 % Detail

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Polymer type: polydeoxyribonucleotide polyribonucleotide

	Total	1H	13C
All	94.0 % (142 of 151)	96.5 % (82 of 85)	90.9 % (60 of 66)
Suger, PO4	93.6 % (103 of 110)	96.7 % (58 of 60)	90.0 % (45 of 50)
Nucleobase	95.1 % (39 of 41)	96.0 % (24 of 25)	93.8 % (15 of 16)
Aromatic	94.6 % (35 of 37)	95.2 % (20 of 21)	93.8 % (15 of 16)

2. entity 2

GUAAGCCGAG

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/RNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	tc-DNA/RNA	natural abundance		0.5 mM
2	tc-DNA/RNA	natural abundance		0.5 mM
3	NaCl	natural abundance		50 mM
4	NaH2PO4	natural abundance		10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/RNA, 50 mM NaCl, 10 mM NaH2PO4, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	tc-DNA/RNA	natural abundance		0.5 mM
6	tc-DNA/RNA	natural abundance		0.5 mM
7	NaCl	natural abundance		50 mM
8	NaH2PO4	natural abundance		10 mM

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Release date

2018-06-03

Citation

NMR solution structure of tricyclo-DNA containing duplexes: insight into enhanced thermal stability and nuclease resistance
Istrate, A., Johannsen, S., Istrate, A., Sigel, R.K.O., Leumann, C.
Nucleic Acids Res. (2019), 47, 4872-4882, PubMed 30916334 , DOI 10.1093/nar/gkz197 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Experiments performed 5 experiments Detail