BMRBj - BMREntryMaster

Found 1 Document (0.352 seconds)

BMRB: 34277

6GN4

tc-DNA/tc-DNA duplex

Authors

Istrate, A., Johannsen, S., Istrate, A., Sigel, R.K.O., Leumann, C.

Assembly

tc-DNA/tc-DNA duplex

Entity

1. tc-DNA/tc-DNA duplex, entity 1 (polymer, Thiol state: not present), 10 monomers, 3495.526 Da
2. tc-DNA/tc-DNA duplex, entity 2 (polymer, Thiol state: not present), 10 monomers, 3565.548 Da

Total weight

7061.074 Da

Max. entity weight

3565.548 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 190 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	TCJ	H1'	H	6.250	0.001	1
2	1	1	TCJ	H2'	H	3.005	0.001	1
3	1	1	TCJ	H2''	H	3.343	0.001	1
4	1	1	TCJ	H4'	H	4.848	0.001	1
5	1	1	TCJ	H6	H	8.124	0.000	1
6	1	1	TCJ	H6'	H	1.947	0.000	1
7	1	1	TCJ	H7'	H	2.148	0.002	1
8	1	1	TCJ	H7''	H	2.564	0.001	1
9	1	1	TCJ	H8'	H	1.202	0.000	1
10	1	1	TCJ	H8''	H	1.847	0.001	1
11	1	2	EAN	H1'	H	6.169	0.002	1
12	1	2	EAN	H2'	H	3.383	0.000	1
13	1	2	EAN	H2''	H	3.347	0.002	1
14	1	2	EAN	H6	H	7.759	0.033	1
15	1	2	EAN	H7'	H	2.026	0.004	1
16	1	2	EAN	H7''	H	2.771	0.001	1
17	1	2	EAN	H71	H	1.849	0.000	1
18	1	2	EAN	H72	H	1.849	0.000	1
19	1	2	EAN	H73	H	1.849	0.000	1
20	1	3	TCJ	H1'	H	6.015	0.002	1
21	1	3	TCJ	H2'	H	3.118	0.000	1
22	1	3	TCJ	H2''	H	3.285	0.001	1
23	1	3	TCJ	H6	H	7.648	0.003	1
24	1	3	TCJ	H7'	H	2.069	0.004	1
25	1	3	TCJ	H7''	H	2.652	0.003	1
26	1	3	TCJ	H41	H	8.901	0.002	1
27	1	3	TCJ	H42	H	6.744	0.007	1
28	1	3	TCJ	H71	H	1.937	0.001	1
29	1	3	TCJ	H72	H	1.937	0.001	1
30	1	3	TCJ	H73	H	1.937	0.001	1
31	1	4	F4Q	H1	H	12.887	0.001	1
32	1	4	F4Q	H1'	H	6.214	0.001	1
33	1	4	F4Q	H2'	H	3.273	0.002	1
34	1	4	F4Q	H2''	H	3.345	0.000	1
35	1	4	F4Q	H7'	H	1.617	0.002	1
36	1	4	F4Q	H7''	H	2.557	0.001	1
37	1	4	F4Q	H8	H	7.735	0.001	1
38	1	5	F4Q	H1	H	13.274	0.002	1
39	1	5	F4Q	H1'	H	6.380	0.001	1
40	1	5	F4Q	H2'	H	3.308	0.001	1
41	1	5	F4Q	H2''	H	3.344	0.001	1
42	1	5	F4Q	H7'	H	1.564	0.001	1
43	1	5	F4Q	H7''	H	2.733	0.002	1
44	1	5	F4Q	H8	H	7.452	0.001	1
45	1	6	TCJ	H1'	H	6.055	0.001	1
46	1	6	TCJ	H2'	H	3.047	0.001	1
47	1	6	TCJ	H2''	H	3.304	0.001	1
48	1	6	TCJ	H4'	H	4.856	0.001	1
49	1	6	TCJ	H6	H	7.347	0.001	1
50	1	6	TCJ	H7'	H	1.900	0.001	1
51	1	6	TCJ	H7''	H	2.740	0.000	1
52	1	6	TCJ	H41	H	8.904	0.001	1
53	1	6	TCJ	H42	H	6.767	0.000	1
54	1	6	TCJ	H71	H	1.572	0.002	1
55	1	6	TCJ	H72	H	1.572	0.002	1
56	1	6	TCJ	H73	H	1.572	0.002	1
57	1	7	EAN	H1'	H	6.098	0.001	1
58	1	7	EAN	H2'	H	3.152	0.003	1
59	1	7	EAN	H2''	H	3.364	0.003	1
60	1	7	EAN	H3	H	14.404	0.001	1
61	1	7	EAN	H4'	H	4.876	0.001	1
62	1	7	EAN	H6	H	7.641	0.001	1
63	1	7	EAN	H7'	H	1.984	0.000	1
64	1	7	EAN	H7''	H	2.814	0.004	1
65	1	7	EAN	H8'	H	1.349	0.001	1
66	1	7	EAN	H8''	H	2.012	0.000	1
67	1	7	EAN	H71	H	1.732	0.000	1
68	1	7	EAN	H72	H	1.732	0.000	1
69	1	7	EAN	H73	H	1.732	0.000	1
70	1	8	EAN	H1'	H	6.020	0.001	1
71	1	8	EAN	H2'	H	2.925	0.001	1
72	1	8	EAN	H2''	H	3.374	0.001	1
73	1	8	EAN	H3	H	13.577	0.002	1
74	1	8	EAN	H6	H	7.723	0.001	1
75	1	8	EAN	H7'	H	2.124	0.001	1
76	1	8	EAN	H7''	H	2.740	0.001	1
77	1	8	EAN	H71	H	1.893	0.003	1
78	1	8	EAN	H72	H	1.893	0.003	1
79	1	8	EAN	H73	H	1.893	0.003	1
80	1	9	TCY	H1'	H	6.572	0.001	1
81	1	9	TCY	H2	H	7.372	0.001	1
82	1	9	TCY	H2'	H	3.285	0.003	1
83	1	9	TCY	H2'A	H	3.461	0.001	1
84	1	9	TCY	H7'	H	1.607	0.000	1
85	1	9	TCY	H7'A	H	2.734	0.002	1
86	1	9	TCY	H8	H	8.174	0.000	1
87	1	10	TCJ	H1'	H	5.923	0.001	1
88	1	10	TCJ	H2'	H	2.443	0.001	1
89	1	10	TCJ	H2''	H	2.802	0.003	1
90	1	10	TCJ	H4'	H	4.724	0.001	1
91	1	10	TCJ	H6	H	7.219	0.001	1
92	1	10	TCJ	H7'	H	2.442	0.001	1
93	1	10	TCJ	H7''	H	2.797	0.000	1
94	1	10	TCJ	H71	H	1.506	0.000	1
95	1	10	TCJ	H72	H	1.506	0.000	1
96	1	10	TCJ	H73	H	1.506	0.000	1
97	2	1	F4Q	H1'	H	6.465	0.001	1
98	2	1	F4Q	H2'	H	3.305	0.001	1
99	2	1	F4Q	H2''	H	3.414	0.001	1
100	2	1	F4Q	H4'	H	4.927	0.000	1
101	2	1	F4Q	6H'	H	1.906	0.000	1
102	2	1	F4Q	H6'	H	1.906	0.001	1
103	2	1	F4Q	H7'	H	1.754	0.003	1
104	2	1	F4Q	H7''	H	2.530	0.002	1
105	2	1	F4Q	H8	H	8.366	0.001	1
106	2	1	F4Q	H8'	H	1.158	0.002	1
107	2	1	F4Q	H8''	H	1.684	0.002	1
108	2	2	EAN	H1'	H	6.202	0.001	1
109	2	2	EAN	H2'	H	3.497	0.001	1
110	2	2	EAN	H2''	H	3.412	0.001	1
111	2	2	EAN	H3	H	13.422	0.004	1
112	2	2	EAN	H6	H	7.597	0.001	1
113	2	2	EAN	H7'	H	2.152	0.001	1
114	2	2	EAN	H7''	H	2.695	0.001	1
115	2	2	EAN	H71	H	1.687	0.000	1
116	2	2	EAN	H72	H	1.687	0.000	1
117	2	2	EAN	H73	H	1.687	0.000	1
118	2	3	TCY	H1'	H	6.535	0.001	1
119	2	3	TCY	H2	H	6.286	0.000	1
120	2	3	TCY	H2'	H	3.382	0.001	1
121	2	3	TCY	H2'A	H	3.568	0.002	1
122	2	3	TCY	H7'	H	1.759	0.003	1
123	2	3	TCY	H7'A	H	2.656	0.001	1
124	2	3	TCY	H8	H	8.323	0.000	1
125	2	4	TCY	H1'	H	6.472	0.003	1
126	2	4	TCY	H2	H	7.401	0.001	1
127	2	4	TCY	H2'	H	3.408	0.001	1
128	2	4	TCY	H2'A	H	3.471	0.004	1
129	2	4	TCY	H7'	H	1.665	0.000	1
130	2	4	TCY	H7'A	H	2.569	0.004	1
131	2	4	TCY	H8	H	7.874	0.001	1
132	2	5	F4Q	H1	H	13.435	0.005	1
133	2	5	F4Q	H1'	H	6.339	0.001	1
134	2	5	F4Q	H2'	H	3.292	0.001	1
135	2	5	F4Q	H2''	H	3.351	0.000	1
136	2	5	F4Q	H7'	H	1.607	0.000	1
137	2	5	F4Q	H7''	H	2.772	0.001	1
138	2	5	F4Q	H8	H	7.385	0.001	1
139	2	6	TCJ	H1'	H	6.050	0.002	1
140	2	6	TCJ	H2'	H	2.961	0.000	1
141	2	6	TCJ	H2''	H	3.299	0.001	1
142	2	6	TCJ	H4'	H	4.854	0.002	1
143	2	6	TCJ	H6	H	7.312	0.001	1
144	2	6	TCJ	H7'	H	1.935	0.003	1
145	2	6	TCJ	H7''	H	2.745	0.004	1
146	2	6	TCJ	H41	H	8.893	0.000	1
147	2	6	TCJ	H42	H	6.753	0.000	1
148	2	6	TCJ	H71	H	1.513	0.001	1
149	2	6	TCJ	H72	H	1.513	0.001	1
150	2	6	TCJ	H73	H	1.513	0.001	1
151	2	7	TCJ	H1'	H	5.993	0.001	1
152	2	7	TCJ	H2'	H	3.136	0.001	1
153	2	7	TCJ	H2''	H	3.299	0.001	1
154	2	7	TCJ	H4'	H	4.864	0.000	1
155	2	7	TCJ	H6	H	7.579	0.001	1
156	2	7	TCJ	H7'	H	2.083	0.001	1
157	2	7	TCJ	H7''	H	2.645	0.002	1
158	2	7	TCJ	H41	H	8.891	0.000	1
159	2	7	TCJ	H42	H	6.684	0.003	1
160	2	7	TCJ	H71	H	1.751	0.001	1
161	2	7	TCJ	H72	H	1.751	0.001	1
162	2	7	TCJ	H73	H	1.751	0.001	1
163	2	8	F4Q	H1	H	12.552	0.003	1
164	2	8	F4Q	H1'	H	6.288	0.001	1
165	2	8	F4Q	H2'	H	3.231	0.001	1
166	2	8	F4Q	H2''	H	3.418	0.001	1
167	2	8	F4Q	H7'	H	1.759	0.001	1
168	2	8	F4Q	H7''	H	2.640	0.003	1
169	2	8	F4Q	H8	H	7.837	0.000	1
170	2	8	F4Q	H21	H	7.635	0.000	1
171	2	9	TCY	H1'	H	6.603	0.001	1
172	2	9	TCY	H2	H	7.563	0.000	1
173	2	9	TCY	H2'	H	3.425	0.000	1
174	2	9	TCY	H2'A	H	3.501	0.002	1
175	2	9	TCY	H6'	H	1.860	0.003	1
176	2	9	TCY	H7'	H	1.771	0.000	1
177	2	9	TCY	H7'A	H	2.560	0.002	1
178	2	9	TCY	H8	H	8.039	0.000	1
179	2	9	TCY	H8'	H	1.245	0.000	1
180	2	9	TCY	H8'A	H	1.862	0.000	1
181	2	9	TCY	HN6	H	8.489	0.001	1
182	2	9	TCY	HN6A	H	6.484	0.000	1
183	2	10	F4Q	H1'	H	6.416	0.001	1
184	2	10	F4Q	H2'	H	2.917	0.002	1
185	2	10	F4Q	H2''	H	2.938	0.001	1
186	2	10	F4Q	H4'	H	4.820	0.000	1
187	2	10	F4Q	H6'	H	1.683	0.000	1
188	2	10	F4Q	H8	H	7.675	0.001	1
189	2	10	F4Q	H8'	H	1.071	0.001	1
190	2	10	F4Q	H8''	H	1.743	0.000	1

Release date

2018-05-31

Citation

NMR solution structure of tricyclo-DNA containing duplexes: insight into enhanced thermal stability and nuclease resistance
Istrate, A., Johannsen, S., Istrate, A., Sigel, R.K.O., Leumann, C.
Nucleic Acids Res. (2019), 47, 4872-4882, PubMed 30916334 , DOI 10.1093/nar/gkz197 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 34280 released on 2018-06-06
    Title Tc-DNA/DNA duplex
  BMRB: 34276 released on 2018-06-03
    Title Tc-DNA/RNA duplex

Experiments performed 6 experiments Detail

1 2D 1H-1H NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/tc-DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/tc-DNA	natural abundance	0.5 mM
2	tc-DNA/tc-DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

2 2D DQF-COSY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/tc-DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/tc-DNA	natural abundance	0.5 mM
2	tc-DNA/tc-DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

3 2D 1H-1H TOCSY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/tc-DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/tc-DNA	natural abundance	0.5 mM
2	tc-DNA/tc-DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

4 2D 1H-13C HSQC

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.05, Details 0.5 mM tc-DNA/tc-DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
1	tc-DNA/tc-DNA	natural abundance	0.5 mM
2	tc-DNA/tc-DNA	natural abundance	0.5 mM
3	NaCl	natural abundance	50 mM
4	NaH2PO4	natural abundance	10 mM

5 2D 1H-1H NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.05, Details 0.5 mM tc-DNA/tc-DNA, 50 mM NaCl, 10 mM NaH2PO4, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	tc-DNA/tc-DNA	natural abundance	0.5 mM
6	tc-DNA/tc-DNA	natural abundance	0.5 mM
7	NaCl	natural abundance	50 mM
8	NaH2PO4	natural abundance	10 mM

6 2D 1H-1H NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.05, Details 0.5 mM tc-DNA/tc-DNA, 50 mM NaCl, 10 mM NaH2PO4, 100% D2O

#	Name	Isotope labeling	Concentration
9	tc-DNA/tc-DNA	natural abundance	0.5 mM
10	tc-DNA/tc-DNA	natural abundance	0.5 mM
11	NaCl	natural abundance	50 mM
12	NaH2PO4	natural abundance	10 mM

NMR combined restraints 2 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts	combined_34277_6gn4.nef
Input source #2: Coordindates	6gn4.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Distance (Å)
1:1:F7H:O3'	1:2:EAN:P	n/a
1:2:EAN:O3'	1:3:TCJ:P	n/a
1:3:TCJ:O3'	1:4:F4Q:P	n/a
1:4:F4Q:O3'	1:5:F4Q:P	n/a
1:5:F4Q:O3'	1:6:TCJ:P	n/a
1:6:TCJ:O3'	1:7:EAN:P	n/a
1:7:EAN:O3'	1:8:EAN:P	n/a
1:8:EAN:O3'	1:9:TCY:P	n/a
1:9:TCY:O3'	1:10:TCJ:P	n/a
2:1:F7K:O3'	2:2:EAN:P	n/a
2:2:EAN:O3'	2:3:TCY:P	n/a
2:3:TCY:O3'	2:4:TCY:P	n/a
2:4:TCY:O3'	2:5:F4Q:P	n/a
2:5:F4Q:O3'	2:6:TCJ:P	n/a
2:6:TCJ:O3'	2:7:TCJ:P	n/a
2:7:TCJ:O3'	2:8:F4Q:P	n/a
2:8:F4Q:O3'	2:9:TCY:P	n/a
2:9:TCY:O3'	2:10:F4Q:P	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
A	1	F7H	4-azanyl-1-[(1~{S},2~{R},4~{S},6~{S},8~{R})-2,6-bis(oxidanyl)-9-oxatricyclo[4.3.0.0^{2,4}]nonan-8-yl]-5-methyl-pyrimidin-2-one	Assigned chemical shifts, Coordinates
A	2	EAN	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
A	3	TCJ	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(4-azanyl-5-methyl-2-oxidanylidene-pyrimidin-1-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
A	4	F4Q	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
A	5	F4Q	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
A	6	TCJ	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(4-azanyl-5-methyl-2-oxidanylidene-pyrimidin-1-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
A	7	EAN	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
A	8	EAN	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
A	9	TCY	(2R,3aS,4aR,5aR,5bS)-2-(6-amino-9H-purin-9-yl)-3a-hydroxyhexahydrocyclopropa[4,5]cyclopenta[1,2-b]furan-5a(4H)-yl dihydrogen phosphate	Assigned chemical shifts, Coordinates
A	10	TCJ	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(4-azanyl-5-methyl-2-oxidanylidene-pyrimidin-1-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
B	11	F7K	2-azanyl-9-[(1~{S},2~{R},4~{S},6~{S},8~{R})-2,6-bis(oxidanyl)-9-oxatricyclo[4.3.0.0^{2,4}]nonan-8-yl]-1~{H}-purin-6-one	Assigned chemical shifts, Coordinates
B	12	EAN	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
B	13	TCY	(2R,3aS,4aR,5aR,5bS)-2-(6-amino-9H-purin-9-yl)-3a-hydroxyhexahydrocyclopropa[4,5]cyclopenta[1,2-b]furan-5a(4H)-yl dihydrogen phosphate	Assigned chemical shifts, Coordinates
B	14	TCY	(2R,3aS,4aR,5aR,5bS)-2-(6-amino-9H-purin-9-yl)-3a-hydroxyhexahydrocyclopropa[4,5]cyclopenta[1,2-b]furan-5a(4H)-yl dihydrogen phosphate	Assigned chemical shifts, Coordinates
B	15	F4Q	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
B	16	TCJ	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(4-azanyl-5-methyl-2-oxidanylidene-pyrimidin-1-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
B	17	TCJ	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(4-azanyl-5-methyl-2-oxidanylidene-pyrimidin-1-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
B	18	F4Q	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates
B	19	TCY	(2R,3aS,4aR,5aR,5bS)-2-(6-amino-9H-purin-9-yl)-3a-hydroxyhexahydrocyclopropa[4,5]cyclopenta[1,2-b]furan-5a(4H)-yl dihydrogen phosphate	Assigned chemical shifts, Coordinates
B	20	F4Q	[(1~{S},2~{R},4~{R},6~{S},8~{R})-8-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-6-oxidanyl-9-oxatricyclo[4.3.0.0^{2,4}]nonan-2-yl] dihydrogen phosphate	Assigned chemical shifts, Coordinates

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10
XXXXXXXXXX
||||||||||
XXXXXXXXXX

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--------20
XXXXXXXXXX
||||||||||
XXXXXXXXXX
--------10

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	10	0	0	100.0
B	B	10	0	0	100.0

Content subtype: combined_34277_6gn4.nef

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	18		No information

Covalent bonds

Saveframe: assembly
- Status: OK

Keywords DNA, Modified nucleic acids, Modified oligonucleotide, Tc-DNA, tricyclo-DNA

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.352 seconds)

BMRB: 34277

Entries sharing articles BMRB: 2 entries Detail

Experiments performed 6 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 2 contents Detail