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BMRB: 34291

6GVU

NMR structure of the DNA-bound helix bundle domain from the functional pRN1 primase

Authors

Boudet, J., Lipps, G., Allain, F.H.-T.

Assembly

functional pRN1 primase/DNA Complex

Entity

1. functional pRN1 primase/DNA Complex, entity 1 (polymer, Thiol state: not present), 9 monomers, 2769.757 Da Detail

CTGTGCTCA

2. functional pRN1 primase/DNA Complex, entity 2 (polymer), 115 monomers, 13503.53 Da Detail

TVVEFEELRK ELVKRDSGKP VEKIKEEICT KSPPKLIKEI ICENKTYADV NIDRSRGDWH VILYLMKHGV TDPDKILELL PRDSKAKENE KWNTQKYFVI TLSKAWSVVK KYLEA

Total weight

16273.287 Da

Max. entity weight

13503.53 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	2:CYS29:SG	2:CYS42:SG

Source organism

Sulfolobus islandicus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 73.7 %, Completeness (bb): 74.3 % Detail

Polymer type: polydeoxyribonucleotide polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.7 % (1124 of 1525)	80.3 % (680 of 847)	60.4 % (338 of 560)	89.8 % (106 of 118)
Backbone	74.3 % (552 of 743)	90.7 % (264 of 291)	54.1 % (185 of 342)	93.6 % (103 of 110)
Sidechain	74.5 % (666 of 894)	74.8 % (416 of 556)	74.8 % (247 of 330)	37.5 % (3 of 8)
Aromatic	70.2 % (80 of 114)	75.8 % (50 of 66)	60.0 % (27 of 45)	100.0 % (3 of 3)
Methyl	87.8 % (122 of 139)	88.7 % (63 of 71)	86.8 % (59 of 68)

1. entity 1

CTGTGCTCA

2. entity 2

TVVEFEELRK ELVKRDSGKP VEKIKEEICT KSPPKLIKEI ICENKTYADV NIDRSRGDWH VILYLMKHGV TDPDKILELL PRDSKAKENE KWNTQKYFVI TLSKAWSVVK KYLEA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 1.0 mM [U-99% 13C; U-99% 15N] DNA-bound helix bundle domain, 1.0 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.7 mM [U-100% 13C] DNA-bound helix bundle domain, 0.7 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 100% D2O

#	Name	Isotope labeling	Concentration
4	DNA-bound helix bundle domain	[U-100% 13C]	0.7 (±0.1) mM
5	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	0.7 (±0.1) mM
6	NaCl	natural abundance	100 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 1.1 mM [U-99% 15N] DNA-bound helix bundle domain, 1.1 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	DNA-bound helix bundle domain	[U-99% 15N]	1.1 (±0.1) mM
8	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.1 (±0.1) mM
9	NaCl	natural abundance	50 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 1.0 mM DNA-bound helix bundle domain, 1.0 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	DNA-bound helix bundle domain	natural abundance	1.0 mM
11	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 mM
12	NaCl	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.23 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.23 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6GVU, Strand ID: B Detail

Release date

2018-10-16

Citation

A Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template
Boudet, J., Devillier, J.C., Wiegand, T., Salmon, L., Meier, B.H., Lipps, G., Allain, F.H.-T.
Cell (2018), 176, 154-166, PubMed 30595448 , DOI 10.1016/j.cell.2018.11.031 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 34287 released on 2018-10-24
    Title NMR structure of the free helix bundle domain from the functional pRN1 primase
  BMRB: 34290 released on 2018-09-25
    Title Hybrid structure of the pRN1 helix bundle domain in complex with DNA and 2 ATP molecules

Related entities 2. functional pRN1 primase/DNA Complex, entity 2, : 1 : 4 entities Detail

Experiments performed 25 experiments Detail

1 3D HNCA

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 1.0 mM [U-99% 13C; U-99% 15N] DNA-bound helix bundle domain, 1.0 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

2 3D HN(CO)CA

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

3 3D HNCACB

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

4 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

5 3D C(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

6 3D H(CCO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

7 3D 1H-15N NOESY

Instrument

Bruker AVANCE III HD - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

8 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE III HD - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

9 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE III HD - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA-bound helix bundle domain	[U-99% 13C; U-99% 15N]	1.0 (±0.1) mM
2	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 (±0.1) mM
3	NaCl	natural abundance	100 mM

10 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 0.7 mM [U-100% 13C] DNA-bound helix bundle domain, 0.7 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 100% D2O

#	Name	Isotope labeling	Concentration
4	DNA-bound helix bundle domain	[U-100% 13C]	0.7 (±0.1) mM
5	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	0.7 (±0.1) mM
6	NaCl	natural abundance	100 mM

11 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 1.1 mM [U-99% 15N] DNA-bound helix bundle domain, 1.1 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	DNA-bound helix bundle domain	[U-99% 15N]	1.1 (±0.1) mM
8	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.1 (±0.1) mM
9	NaCl	natural abundance	50 mM

12 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 1.1 mM [U-99% 15N] DNA-bound helix bundle domain, 1.1 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	DNA-bound helix bundle domain	[U-99% 15N]	1.1 (±0.1) mM
8	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.1 (±0.1) mM
9	NaCl	natural abundance	50 mM

13 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.1 mM [U-99% 15N] DNA-bound helix bundle domain, 1.1 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	DNA-bound helix bundle domain	[U-99% 15N]	1.1 (±0.1) mM
8	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.1 (±0.1) mM
9	NaCl	natural abundance	50 mM

14 3D 1H-15N NOESY

Instrument

Bruker AVANCE III HD - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DNA-bound helix bundle domain	[U-99% 15N]	1.1 (±0.1) mM
8	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.1 (±0.1) mM
9	NaCl	natural abundance	50 mM

15 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DNA-bound helix bundle domain	[U-99% 15N]	1.1 (±0.1) mM
8	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.1 (±0.1) mM
9	NaCl	natural abundance	50 mM

16 2D 1H-1H NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 5.5, Details 1.0 mM DNA-bound helix bundle domain, 1.0 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	DNA-bound helix bundle domain	natural abundance	1.0 mM
11	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 mM
12	NaCl	natural abundance	50 mM

17 2D 1H-1H NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 1.0 mM DNA-bound helix bundle domain, 1.0 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	DNA-bound helix bundle domain	natural abundance	1.0 mM
11	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 mM
12	NaCl	natural abundance	50 mM

18 2D 1H-1H TOCSY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
10	DNA-bound helix bundle domain	natural abundance	1.0 mM
11	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 mM
12	NaCl	natural abundance	50 mM

19 2D 1H-1H TOCSY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
10	DNA-bound helix bundle domain	natural abundance	1.0 mM
11	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	1.0 mM
12	NaCl	natural abundance	50 mM

20 2D-F1fF2f-NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	DNA-bound helix bundle domain	[U-100% 13C]	0.7 (±0.1) mM
5	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	0.7 (±0.1) mM
6	NaCl	natural abundance	100 mM

21 2D-F2f-NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	DNA-bound helix bundle domain	[U-100% 13C]	0.7 (±0.1) mM
5	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	0.7 (±0.1) mM
6	NaCl	natural abundance	100 mM

22 3D 13C-aliphatic Hfiltered-Hedited NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	DNA-bound helix bundle domain	[U-100% 13C]	0.7 (±0.1) mM
5	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	0.7 (±0.1) mM
6	NaCl	natural abundance	100 mM

23 3D 13C-aromatic Hfiltered-Hedited NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	DNA-bound helix bundle domain	[U-100% 13C]	0.7 (±0.1) mM
5	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	0.7 (±0.1) mM
6	NaCl	natural abundance	100 mM

24 3D 13C-aliphatic Hfiltered-Hedited NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.7 mM [U-100% 13C] DNA-bound helix bundle domain, 0.7 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 100% D2O

#	Name	Isotope labeling	Concentration
4	DNA-bound helix bundle domain	[U-100% 13C]	0.7 (±0.1) mM
5	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	0.7 (±0.1) mM
6	NaCl	natural abundance	100 mM

25 3D 13C-aromatic Hfiltered-Hedited NOESY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
4	DNA-bound helix bundle domain	[U-100% 13C]	0.7 (±0.1) mM
5	DNA (5'-D(CPTPGPTPGPCPTPCP*A)-3')	natural abundance	0.7 (±0.1) mM
6	NaCl	natural abundance	100 mM

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_34291_6gvu.nef
Input source #2: Coordindates	6gvu.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
B:284:CYS:SG	B:297:CYS:SG	oxidized, CA 58.456, CB 40.998 ppm	oxidized, CB 39.071 ppm	2.039

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---------
CTGTGCTCA
|||||||||
CTGTGCTCA

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--260-------270-------280-------290-------300-------310-------320-------330-------340-------350-----
TVVEFEELRKELVKRDSGKPVEKIKEEICTKSPPKLIKEIICENKTYADVNIDRSRGDWHVILYLMKHGVTDPDKILELLPRDSKAKENEKWNTQKYFVI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
TVVEFEELRKELVKRDSGKPVEKIKEEICTKSPPKLIKEIICENKTYADVNIDRSRGDWHVILYLMKHGVTDPDKILELLPRDSKAKENEKWNTQKYFVI
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

--360-------370
TLSKAWSVVKKYLEA
|||||||||||||||
TLSKAWSVVKKYLEA
-------110-----

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	9	0	0	100.0
B	B	115	0	0	100.0

Content subtype: combined_34291_6gvu.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	1		No information
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1066		100.0 (chain: A, length: 9), 88.7 (chain: B, length: 115)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	4718 (1)	noe	100.0 (chain: A, length: 9), 88.7 (chain: B, length: 115)
2	Distance restraints	DYANA/DIANA_distance_constraints_3	Warning	216 (1)	hbond	67.0 (chain: B, length: 115)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_4	OK	191 (191)	.	100.0 (chain: A, length: 9), 96.5 (chain: B, length: 115)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 9, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 115, Sequence coverage: 88.7%
- Total number of assignments
  - ¹H chemical shifts: 659
  - ¹³C chemical shifts: 303
  - ¹⁵N chemical shifts: 104
- Completeness of assignments
  - Entity_assemble_ID: A
  - Entity_assemble_ID: B
- Redox state of CYS
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	90	76	84.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	63	61	96.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	15	55.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	3	3	100.0
¹³C chemical shifts	3	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	3	75.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	757	583	77.0
¹³C chemical shifts	560	303	54.1
¹⁵N chemical shifts	123	104	84.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	228	197	86.4
¹³C chemical shifts	230	66	28.7
¹⁵N chemical shifts	110	101	91.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	529	386	73.0
¹³C chemical shifts	330	237	71.8
¹⁵N chemical shifts	13	3	23.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	69	57	82.6
¹³C chemical shifts	69	57	82.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	48	38	79.2
¹³C chemical shifts	45	27	60.0
¹⁵N chemical shifts	3	3	100.0

Covalent bonds

Saveframe: assembly
- Status: OK

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 9, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 115, Sequence coverage: 88.7%
- Total number of restraints: 4712
- Weight of restraints: 1.0 (4712)
- Potential type of restraints: upper-bound-parabolic (4712)
- Range of distance target values: 0.0, 6.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: DYANA/DIANA_distance_constraints_3
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: B, Chain length: 115, Sequence coverage: 67.0%
  - Total number of restraints: 216
  - Weight of restraints: 1.0 (216)
  - Potential type of restraints: upper-bound-parabolic (216)
  - Range of distance target values: 1.8, 3.4 (Å)
  - Histogram of distance target values
  - Histogram of discrepancy in distance target values
  - Distance restraints per residue
    - Chain_ID: A
      None
    - Chain_ID: B
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 9, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 115, Sequence coverage: 96.5%
- Total number of restraints: 191
- Total number of restraints per polymer type: 191
- Weight of restraints: 1.0 (191)
- Potential type of restraints: square-well-parabolic (191)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

Keywords DNA BINDING PROTEIN, primase, replication initiation, single-stranded DNA

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Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.77 seconds)

BMRB: 34291

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 2 entries Detail

Related entities 2. functional pRN1 primase/DNA Complex, entity 2, : 1 : 4 entities Detail

Experiments performed 25 experiments Detail

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