BMRBj - BMREntryMaster

Found 1 Document (4.226 seconds)

BMRB: 34461

6TL0

Solution structure and 1H, 13C and 15N chemical shift assignments for the complex of VPS29 with VARP 687-747

Authors

Owen, D.J., Neuhaus, D., Yang, J., Crawley-Snowdon, H.

Assembly

Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27

Entity

1. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 1 (polymer), 192 monomers, 21598.57 Da Detail

GSPEFGTRDR MLVLVLGDLH IPHRCNSLPA KFKKLLVPGK IQHILCTGNL CTKESYDYLK TLAGDVHIVR GDFDENLNYP EQKVVTVGQF KIGLIHGHQV IPWGDMASLA LLQRQFDVDI LISGHTHKFE AFEHENKFYI NPGSATGAYN ALETNIIPSF VLMDIQASTV VTYVYQLIGD DVKVERIEYK KS

2. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 2 (polymer, Thiol state: all other bound), 61 monomers, 6314.886 Da Detail

GPLGSTEEDL EDAEDTVSAA DPEFCHPLCQ CPKCAPAQKR LAKVPASGLG VNVTSQDGSS W

3. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity ZN (non-polymer), 65.409 Da

Total weight

27978.865 Da

Max. entity weight

21598.57 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:CYS25:SG	3:ZN1:ZN
2	na	sing	2:CYS29:SG	3:ZN1:ZN
3	na	sing	2:CYS31:SG	3:ZN1:ZN
4	na	sing	2:CYS34:SG	3:ZN1:ZN

Source organism

Mus musculus , Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 75.5 %, Completeness (bb): 79.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.5 % (2203 of 2918)	78.5 % (1180 of 1504)	68.0 % (779 of 1146)	91.0 % (244 of 268)
Backbone	79.9 % (1190 of 1490)	93.6 % (479 of 512)	65.0 % (480 of 739)	96.7 % (231 of 239)
Sidechain	73.9 % (1228 of 1661)	70.7 % (701 of 992)	80.3 % (514 of 640)	44.8 % (13 of 29)
Aromatic	57.0 % (130 of 228)	69.3 % (79 of 114)	43.8 % (49 of 112)	100.0 % (2 of 2)
Methyl	93.6 % (279 of 298)	93.3 % (139 of 149)	94.0 % (140 of 149)

1. entity 1

2. entity 2

GPLGSTEEDL EDAEDTVSAA DPEFCHPLCQ CPKCAPAQKR LAKVPASGLG VNVTSQDGSS W

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 100% D2O

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 100% D2O

#	Name	Isotope labeling	Concentration
16	VPS29	natural abundance	0.45 (±0.15) mM
17	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
18	TRIS	[U-2H]	20 mM
19	sodium chloride	natural abundance	200 mM
20	DTT	[U-2H]	1 mM

Protein Blocks Logo

Calculated from 25 models in PDB: 6TL0, Strand ID: A, B Detail

Release date

2020-01-06

Citation

Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29
Crawley-Snowdon, H., Yang, J.C., Zaccai, N.R., Davis, L.J., Wartosch, L., Herman, E.K., Bright, N.A., Swarbrick, J.S., Collins, B.M., Jackson, L.P., Seaman, M., Luzio, J.P., Dacks, J.B., Neuhaus, D., Owen, D.J.
Nat. Commun. (2020), 11, 5031-5031, PubMed 33024112 , DOI 10.1038/s41467-020-18773-2 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 50107 released on 2019-12-01
    Title 1H, 13C and 15N chemical shift assignments for VPS29
  BMRB: 50108 released on 2019-12-01
    Title 1H, 13C and 15N chemical shift assignments for VARP 687-747

Related entities 1. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 1, : 6 : 25 entities Detail

Related entities 2. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 2, : 1 : 4 : 6 entities Detail

Interaction partners 1. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 1, : 15 interactors Detail

More details for 15 interactors identified by 4 citations

Interaction partners 2. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 2, : 13 interactors Detail

More details for 13 interactors identified by 7 citations

Experiments performed 54 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

4 2D 1H-13C HSQC aliphatic constant-time

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

5 2D 1H-1H NOESY (150ms mixing time) filtered to reject 13C-1 and 15N-coupled 1H in F1 and to accept 13C- and 15N-coupled 1H in F2

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

6 3D 13C NOESY-HSQC aromatic (150ms mixing time)

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

7 3D 13C NOESY-HSQC aliphatic (150ms mixing time)

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

8 3D 15N NOESY-HSQC (150ms mixing time)

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

9 3D HCCH-TOCSY [13C-13C-1H]

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

10 3D HCCH-TOCSY [1H-13C-1H]

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

11 3D HBHA(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

12 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

13 3D CBCANH

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

14 3D HN(CO)CA

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

15 3D HNCA

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
2	VARP 692-746	natural abundance	0.45 (±0.15) mM
3	TRIS	[U-2H]	20 mM
4	sodium chloride	natural abundance	200 mM
5	DTT	[U-2H]	1 mM

16 2D 1H-1H NOESY (150ms mixing time) filtered to reject 13C-1 and 15N-coupled 1H in F1 and to accept 13C- and 15N-coupled 1H in F2

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.45 mM [U-98% 13C; U-98% 15N] VPS29, 0.45 mM VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 100% D2O

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

17 2D 1H-13C HSQC aliphatic constant-time

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

18 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

19 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

20 2D 1H-1H NOESY (70ms mixing time) filtered to reject 13C-1 and 15N-coupled 1H in F1 and to accept 13C- and 15N-coupled 1H in F2

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

21 3D 13C NOESY-HSQC (120ms mixing time) aromatic, filtered to reject 13C-1 and 15N-coupled 1H in F1

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

22 3D 13C NOESY-HSQC (50ms mixing time) aromatic, filtered to reject 13C-1 and 15N-coupled 1H in F1

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

23 3D 13C NOESY-HSQC (120ms mixing time) aliphatic, filtered to reject 13C-1 and 15N-coupled 1H in F1

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

24 3D 13C NOESY-HSQC (50ms mixing time) aliphatic, filtered to reject 13C-1 and 15N-coupled 1H in F1

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

25 3D 13C NOESY-HSQC (120ms mixing time) aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

26 3D 13C NOESY-HSQC (50ms mixing time) aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

27 3D 13C NOESY-HSQC (120ms mixing time) aliphatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

28 3D 13C NOESY-HSQC (50ms mixing time) aliphatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

29 3D HCCH-TOCSY [13C-13C-1H]

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

30 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

31 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 K, pH 7.0, Details 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

32 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 275 K, pH 7.0, Details 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

33 2D 1H-13C HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

34 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

35 3D HNCA

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

36 3D HN(CO)CA

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

37 3D HBHA(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

38 3D HNHA

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

39 3D HCCH-TOCSY [1H-13C-1H]

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

40 3D HCCH-TOCSY [13C-13C-1H]

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

41 3D 1H-15N NOESY-HSQC (70ms mixing time)

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

42 3D 1H-15N NOESY-HSQC (150ms mixing time)

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

43 3D 1H-15N NOESY-HSQC (50ms mixing time)

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

44 3D 1H-15N NOESY-HSQC (50ms mixing time)

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

45 3D 1H-13C NOESY (70ms mixing time) aliphatic

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	VPS29	natural abundance	0.45 (±0.15) mM
12	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
13	TRIS	[U-2H]	20 mM
14	sodium chloride	natural abundance	200 mM
15	DTT	[U-2H]	1 mM

46 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.45 mM VPS29, 0.45 mM [U-98% 13C; U-98% 15N] VARP 692-746, 20 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM [U-2H] DTT, 100% D2O

#	Name	Isotope labeling	Concentration
16	VPS29	natural abundance	0.45 (±0.15) mM
17	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
18	TRIS	[U-2H]	20 mM
19	sodium chloride	natural abundance	200 mM
20	DTT	[U-2H]	1 mM

47 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
16	VPS29	natural abundance	0.45 (±0.15) mM
17	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
18	TRIS	[U-2H]	20 mM
19	sodium chloride	natural abundance	200 mM
20	DTT	[U-2H]	1 mM

48 3D HCCH-TOCSY [1H-13C-1H]

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
16	VPS29	natural abundance	0.45 (±0.15) mM
17	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
18	TRIS	[U-2H]	20 mM
19	sodium chloride	natural abundance	200 mM
20	DTT	[U-2H]	1 mM

49 3D HCCH-TOCSY [13C-13C-1H]

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
16	VPS29	natural abundance	0.45 (±0.15) mM
17	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
18	TRIS	[U-2H]	20 mM
19	sodium chloride	natural abundance	200 mM
20	DTT	[U-2H]	1 mM

50 3D 13C NOESY-HSQC aliphatic (50ms mixing time)

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
16	VPS29	natural abundance	0.45 (±0.15) mM
17	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
18	TRIS	[U-2H]	20 mM
19	sodium chloride	natural abundance	200 mM
20	DTT	[U-2H]	1 mM

51 3D 13C NOESY-HSQC aliphatic (70ms mixing time)

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
16	VPS29	natural abundance	0.45 (±0.15) mM
17	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
18	TRIS	[U-2H]	20 mM
19	sodium chloride	natural abundance	200 mM
20	DTT	[U-2H]	1 mM

52 3D 13C NOESY-HSQC aliphatic (120ms mixing time)

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
16	VPS29	natural abundance	0.45 (±0.15) mM
17	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
18	TRIS	[U-2H]	20 mM
19	sodium chloride	natural abundance	200 mM
20	DTT	[U-2H]	1 mM

53 3D 13C NOESY-HSQC aromatic (70ms mixing time)

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
16	VPS29	natural abundance	0.45 (±0.15) mM
17	VARP 692-746	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
18	TRIS	[U-2H]	20 mM
19	sodium chloride	natural abundance	200 mM
20	DTT	[U-2H]	1 mM

54 2D 1H-13C HSQC aromatic constant-time

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	VPS29	[U-98% 13C; U-98% 15N]	0.45 (±0.15) mM
7	VARP 692-746	natural abundance	0.45 (±0.15) mM
8	TRIS	[U-2H]	20 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	[U-2H]	1 mM

Chemical shift validation 5 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34461_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
2:25:CYS:SG	3:1:ZN:ZN	reduced, CA 62.064, CB 31.639 ppm	unknown	n/a
2:29:CYS:SG	3:1:ZN:ZN	reduced, CA 62.15, CB 30.88 ppm	unknown	n/a
2:31:CYS:SG	3:1:ZN:ZN	reduced, CA 59.037, CB 27.425 ppm	unknown	n/a
2:34:CYS:SG	3:1:ZN:ZN	reduced, CA 61.982, CB 31.85 ppm	unknown	n/a

Non-standard residues

None

Content subtype: bmr34461_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	2435		98.4 (chain: 1, length: 192), 98.4 (chain: 2, length: 61)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 192, Sequence coverage: 98.4%
  - Entity_assembly_ID: 2, Chain length: 61, Sequence coverage: 98.4%
- Total number of assignments
  - ¹H chemical shifts: 1447
  - ¹³C chemical shifts: 749
  - ¹⁵N chemical shifts: 239
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Keywords INTRACELLULAR VESICLE TRAFFICKING, NMR complex structure, VARP, Zinc finger, endosome, retromer

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1175	915	77.9
¹³C chemical shifts	899	601	66.9
¹⁵N chemical shifts	207	182	87.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	391	370	94.6
¹³C chemical shifts	384	186	48.4
¹⁵N chemical shifts	184	175	95.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	784	545	69.5
¹³C chemical shifts	515	415	80.6
¹⁵N chemical shifts	23	7	30.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	124	118	95.2
¹³C chemical shifts	124	119	96.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	101	70	69.3
¹³C chemical shifts	100	39	39.0
¹⁵N chemical shifts	1	1	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	329	250	76.0
¹³C chemical shifts	247	148	59.9
¹⁵N chemical shifts	61	57	93.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	121	109	90.1
¹³C chemical shifts	122	59	48.4
¹⁵N chemical shifts	55	52	94.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	208	141	67.8
¹³C chemical shifts	125	89	71.2
¹⁵N chemical shifts	6	5	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	13	3	23.1
¹³C chemical shifts	12	2	16.7
¹⁵N chemical shifts	1	1	100.0

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (4.226 seconds)

BMRB: 34461

Sample #1

Sample #2

Sample #3

Sample #4

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 1, : 6 : 25 entities Detail

Related entities 2. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 2, : 1 : 4 : 6 entities Detail

Interaction partners 1. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 1, : 15 interactors Detail

Interaction partners 2. Vacuolar protein sorting-associated protein 29, Ankyrin repeat domain-containing protein 27, entity 2, : 13 interactors Detail

Experiments performed 54 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument