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Found 1 Document (0.828 seconds)

BMRB: 34535

6ZV4

Human TFIIS N-terminal domain (TND)

Authors

Veverka, V.

Assembly

Transcription elongation factor A protein 1

Entity

1. Transcription elongation factor A protein 1 (polymer, Thiol state: not present), 81 monomers, 9049.577 Da Detail

MEDEVVRFAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV NAIRKQSTDE EVTSLAKSLI KSWKKLLDGG S

Formula weight

9049.577 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 96.4 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.4 % (936 of 971)	97.1 % (495 of 510)	96.2 % (357 of 371)	93.3 % (84 of 90)
Backbone	96.3 % (466 of 484)	95.2 % (157 of 165)	97.1 % (232 of 239)	96.2 % (77 of 80)
Sidechain	96.8 % (546 of 564)	98.0 % (338 of 345)	96.2 % (201 of 209)	70.0 % (7 of 10)
Aromatic	90.9 % (20 of 22)	100.0 % (11 of 11)	80.0 % (8 of 10)	100.0 % (1 of 1)
Methyl	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)

1. entity 1

MEDEVVRFAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV NAIRKQSTDE EVTSLAKSLI KSWKKLLDGG S

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] TFIIS, 25 mM deuterated TRIS, 200 mM sodium phospate, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	TFIIS	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium phospate	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

Release date

2020-08-16

Citation

A ubiquitous disordered protein interaction module orchestrates transcription elongation
Cermakova, K., Demeulemeester, J., Lux, V., Nedomova, M., Goldman, S.R., Smith, E.A., Srb, P., Hexnerova, R., Fabry, M., Madlikova, M., Horejsi, M., De Rijck, J., Debyser, Z., Adelman, K., Hodges, H.C., Veverka, V.
Science (2021), 374, 1113-1121, PubMed 34822292 , DOI 10.1126/science.abe2913 , Abstract

Entries sharing articles BMRB: 6 entries Detail

  BMRB: 34536 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human Elongin-A
  BMRB: 34537 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human LEDGF/p75
  BMRB: 34538 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human IWS1
  BMRB: 34539 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human PPP1R10
  BMRB: 34540 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human MED26
  BMRB: 34541 released on 2020-07-26
    Title TFIIS N-terminal domain (TND) from human IWS1

Related entities 1. Transcription elongation factor A protein 1, : 1 : 1 : 12 : 54 entities Detail

Interaction partners 1. Transcription elongation factor A protein 1, : 13 interactors Detail

More details for 13 interactors identified by 7 citations

Experiments performed 7 experiments Detail

1 3D HNCACB

Instrument

Bruker AVANCE - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] TFIIS, 25 mM deuterated TRIS, 200 mM sodium phospate, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	TFIIS	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium phospate	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

2 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TFIIS	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium phospate	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

3 3D HNCO

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TFIIS	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium phospate	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

4 3D HBHA(CO)NH

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TFIIS	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium phospate	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

5 3D HCCH-TOCSY

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TFIIS	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium phospate	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

6 3D 1H-15N NOESY

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TFIIS	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium phospate	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

7 3D 1H-13C NOESY

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	TFIIS	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium phospate	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34535_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34535_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1049		97.5 (chain: 1, length: 81)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 81, Sequence coverage: 97.5%
- Total number of assignments
  - ¹H chemical shifts: 609
  - ¹³C chemical shifts: 357
  - ¹⁵N chemical shifts: 83
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
37	THR	HG1	5.669

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	510	498	97.6
¹³C chemical shifts	371	357	96.2
¹⁵N chemical shifts	90	83	92.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	165	158	95.8
¹³C chemical shifts	162	156	96.3
¹⁵N chemical shifts	80	76	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	345	340	98.6
¹³C chemical shifts	209	201	96.2
¹⁵N chemical shifts	10	7	70.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	55	55	100.0
¹³C chemical shifts	55	55	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	11	11	100.0
¹³C chemical shifts	10	8	80.0
¹⁵N chemical shifts	1	1	100.0

Keywords TRANSCRIPTION, elongation factor

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Found 1 Document (0.828 seconds)

BMRB: 34535

Sample

Entries sharing articles BMRB: 6 entries Detail

Related entities 1. Transcription elongation factor A protein 1, : 1 : 1 : 12 : 54 entities Detail

Interaction partners 1. Transcription elongation factor A protein 1, : 13 interactors Detail

Experiments performed 7 experiments Detail

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Chemical shift validation 3 contents Detail