BMRBj - BMREntryMaster

Found 1 Document (0.831 seconds)

BMRB: 34536

6ZUZ

TFIIS N-terminal domain (TND) from human Elongin-A

Authors

Veverka, V.

Assembly

Elongin-A

Entity

1. Elongin-A (polymer, Thiol state: not present), 90 monomers, 9689.155 Da Detail

SNAASAMAAE SALQVVEKLQ ARLAANPDPK KLLKYLKKLS TLPITVDILA ETGVGKTVNS LRKHEHVGSF ARDLVAQWKK LVPVERNAGS

Formula weight

9689.155 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.3 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.3 % (1012 of 1040)	97.8 % (526 of 538)	97.8 % (395 of 404)	92.9 % (91 of 98)
Backbone	97.0 % (516 of 532)	97.8 % (176 of 180)	97.0 % (258 of 266)	95.3 % (82 of 86)
Sidechain	96.6 % (574 of 594)	97.5 % (349 of 358)	96.9 % (217 of 224)	66.7 % (8 of 12)
Aromatic	89.5 % (34 of 38)	100.0 % (19 of 19)	77.8 % (14 of 18)	100.0 % (1 of 1)
Methyl	98.4 % (124 of 126)	98.4 % (62 of 63)	98.4 % (62 of 63)

1. entity 1

SNAASAMAAE SALQVVEKLQ ARLAANPDPK KLLKYLKKLS TLPITVDILA ETGVGKTVNS LRKHEHVGSF ARDLVAQWKK LVPVERNAGS

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] ELOA, 25 mM deuterated TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	ELOA	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

Release date

2020-08-16

Citation

A ubiquitous disordered protein interaction module orchestrates transcription elongation
Cermakova, K., Demeulemeester, J., Lux, V., Nedomova, M., Goldman, S.R., Smith, E.A., Srb, P., Hexnerova, R., Fabry, M., Madlikova, M., Horejsi, M., De Rijck, J., Debyser, Z., Adelman, K., Hodges, H.C., Veverka, V.
Science (2021), 374, 1113-1121, PubMed 34822292 , DOI 10.1126/science.abe2913 , Abstract

Entries sharing articles BMRB: 6 entries Detail

  BMRB: 34535 released on 2020-08-16
    Title Human TFIIS N-terminal domain (TND)
  BMRB: 34537 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human LEDGF/p75
  BMRB: 34538 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human IWS1
  BMRB: 34539 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human PPP1R10
  BMRB: 34540 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human MED26
  BMRB: 34541 released on 2020-07-26
    Title TFIIS N-terminal domain (TND) from human IWS1

Related entities 1. Elongin-A, : 1 : 49 entities Detail

Interaction partners 1. Elongin-A, : 19 interactors Detail

More details for 19 interactors identified by 7 citations

Experiments performed 7 experiments Detail

1 3D HNCACB

Instrument

Bruker AVANCE - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] ELOA, 25 mM deuterated TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	ELOA	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

2 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ELOA	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

3 3D HNCO

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ELOA	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

4 3D HBHA(CO)NH

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ELOA	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

5 3D HCCH-TOCSY

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ELOA	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

6 3D 1H-13C NOESY

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ELOA	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

7 3D 1H-15N NOESY

Instrument

Bruker AVANCE - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ELOA	[U-13C; U-15N]	0.5 mM
2	TRIS	deuterated	25 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34536_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34536_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	1140		100.0 (chain: 1, length: 90)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 90, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 654
  - ¹³C chemical shifts: 395
  - ¹⁵N chemical shifts: 91
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	538	526	97.8
¹³C chemical shifts	404	395	97.8
¹⁵N chemical shifts	98	91	92.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	180	176	97.8
¹³C chemical shifts	180	177	98.3
¹⁵N chemical shifts	86	83	96.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	358	350	97.8
¹³C chemical shifts	224	218	97.3
¹⁵N chemical shifts	12	8	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	64	64	100.0
¹³C chemical shifts	64	64	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	19	100.0
¹³C chemical shifts	18	14	77.8
¹⁵N chemical shifts	1	1	100.0

Keywords TRANSCRIPTION, elongation factor

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Found 1 Document (0.831 seconds)

BMRB: 34536

Sample

Entries sharing articles BMRB: 6 entries Detail

Related entities 1. Elongin-A, : 1 : 49 entities Detail

Interaction partners 1. Elongin-A, : 19 interactors Detail

Experiments performed 7 experiments Detail

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Chemical shift validation 3 contents Detail