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BMRB: 34540

6ZV3

TFIIS N-terminal domain (TND) from human MED26

Authors

Assembly

Mediator of RNA polymerase II transcription subunit 26

Entity

1. Mediator of RNA polymerase II transcription subunit 26 (polymer, Thiol state: not present), 90 monomers, 10166.73 Da Detail

SMTAAPASPQ QIRDRLLQAI DPQSNIRNMV AVLEVISSLE KYPITKEALE ETRLGKLIND VRKKTKNEEL AKRAKKLLRS WQKLIEPAGS

Formula weight

10166.73 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 93.2 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.2 % (1019 of 1093)	93.6 % (540 of 577)	94.2 % (390 of 414)	87.3 % (89 of 102)
Backbone	95.8 % (508 of 530)	95.5 % (169 of 177)	96.3 % (258 of 268)	95.3 % (81 of 85)
Sidechain	91.7 % (597 of 651)	92.8 % (371 of 400)	93.2 % (218 of 234)	47.1 % (8 of 17)
Aromatic	90.0 % (18 of 20)	100.0 % (10 of 10)	77.8 % (7 of 9)	100.0 % (1 of 1)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. entity 1

SMTAAPASPQ QIRDRLLQAI DPQSNIRNMV AVLEVISSLE KYPITKEALE ETRLGKLIND VRKKTKNEEL AKRAKKLLRS WQKLIEPAGS

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] MED26, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MED26	[U-13C; U-15N]		0.5 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

Release date

2020-08-16

Citation

A ubiquitous disordered protein interaction module orchestrates transcription elongation
Cermakova, K., Demeulemeester, J., Lux, V., Nedomova, M., Goldman, S.R., Smith, E.A., Srb, P., Hexnerova, R., Fabry, M., Madlikova, M., Horejsi, M., De Rijck, J., Debyser, Z., Adelman, K., Hodges, H.C., Veverka, V.
Science (2021), 374, 1113-1121, PubMed 34822292 , DOI 10.1126/science.abe2913 , Abstract

Entries sharing articles BMRB: 6 entries Detail

  BMRB: 34535 released on 2020-08-16
    Title Human TFIIS N-terminal domain (TND)
  BMRB: 34536 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human Elongin-A
  BMRB: 34537 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human LEDGF/p75
  BMRB: 34538 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human IWS1
  BMRB: 34539 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human PPP1R10
  BMRB: 34541 released on 2020-07-26
    Title TFIIS N-terminal domain (TND) from human IWS1

Related entities 1. Mediator of RNA polymerase II transcription subunit 26, : 1 : 8 : 43 entities Detail

Interaction partners 1. Mediator of RNA polymerase II transcription subunit 26, : 29 interactors Detail

More details for 29 interactors identified by 8 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] MED26, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MED26	[U-13C; U-15N]		0.5 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] MED26, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MED26	[U-13C; U-15N]		0.5 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

3 3D HNCACB

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] MED26, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MED26	[U-13C; U-15N]		0.5 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

4 3D CBCA(CO)NH

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] MED26, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MED26	[U-13C; U-15N]		0.5 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

5 3D HNCO

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] MED26, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MED26	[U-13C; U-15N]		0.5 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

6 3D HCCH-TOCSY

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] MED26, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MED26	[U-13C; U-15N]		0.5 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

7 3D HBHA(CO)NH

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.5 mM [U-13C; U-15N] MED26, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	MED26	[U-13C; U-15N]		0.5 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34540_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34540_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1138		97.8 (chain: 1, length: 90)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 90, Sequence coverage: 97.8%
- Total number of assignments
  - ¹H chemical shifts: 659
  - ¹³C chemical shifts: 390
  - ¹⁵N chemical shifts: 89
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords TRANSCRIPTION, transcription elongation