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BMRB: 34541

6ZV4

TFIIS N-terminal domain (TND) from human IWS1

Authors

Assembly

Transcription elongation factor A protein 1,Protein IWS1 homolog

Entity

1. Transcription elongation factor A protein 1,Protein IWS1 homolog (polymer, Thiol state: not present), 116 monomers, 12582.01 Da Detail

MEDEVVRFAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV NAIRKQSTDE EVTSLAKSLI KSWKKLLDGG SGSGSGSGSS GELSDKKNEE KDLFGSDSES GNEEEN

Formula weight

12582.01 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.4 %, Completeness: 88.6 %, Completeness (bb): 88.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.6 % (1166 of 1316)	87.3 % (604 of 692)	90.9 % (451 of 496)	86.7 % (111 of 128)
Backbone	88.2 % (612 of 694)	84.3 % (204 of 242)	90.5 % (305 of 337)	89.6 % (103 of 115)
Sidechain	89.7 % (652 of 727)	88.9 % (400 of 450)	92.4 % (244 of 264)	61.5 % (8 of 13)
Aromatic	87.5 % (28 of 32)	100.0 % (16 of 16)	73.3 % (11 of 15)	100.0 % (1 of 1)
Methyl	98.1 % (106 of 108)	96.3 % (52 of 54)	100.0 % (54 of 54)

1. entity 1

MEDEVVRFAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV NAIRKQSTDE EVTSLAKSLI KSWKKLLDGG SGSGSGSGSS GELSDKKNEE KDLFGSDSES GNEEEN

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.35 mM [U-13C; U-15N] TFIIS-IWS1, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIS-IWS1	[U-13C; U-15N]		0.35 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

Release date

2020-07-26

Citation

A ubiquitous disordered protein interaction module orchestrates transcription elongation
Cermakova, K., Demeulemeester, J., Lux, V., Nedomova, M., Goldman, S.R., Smith, E.A., Srb, P., Hexnerova, R., Fabry, M., Madlikova, M., Horejsi, M., De Rijck, J., Debyser, Z., Adelman, K., Hodges, H.C., Veverka, V.
Science (2021), 374, 1113-1121, PubMed 34822292 , DOI 10.1126/science.abe2913 , Abstract

Entries sharing articles BMRB: 6 entries Detail

  BMRB: 34535 released on 2020-08-16
    Title Human TFIIS N-terminal domain (TND)
  BMRB: 34536 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human Elongin-A
  BMRB: 34537 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human LEDGF/p75
  BMRB: 34538 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human IWS1
  BMRB: 34539 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human PPP1R10
  BMRB: 34540 released on 2020-08-16
    Title TFIIS N-terminal domain (TND) from human MED26

Related entities 1. Transcription elongation factor A protein 1,Protein IWS1 homolog, : 1 : 14 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.35 mM [U-13C; U-15N] TFIIS-IWS1, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIS-IWS1	[U-13C; U-15N]		0.35 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.35 mM [U-13C; U-15N] TFIIS-IWS1, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIS-IWS1	[U-13C; U-15N]		0.35 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

3 3D HNCACB

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.35 mM [U-13C; U-15N] TFIIS-IWS1, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIS-IWS1	[U-13C; U-15N]		0.35 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

4 3D CBCA(CO)NH

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.35 mM [U-13C; U-15N] TFIIS-IWS1, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIS-IWS1	[U-13C; U-15N]		0.35 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

5 3D HNCO

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.35 mM [U-13C; U-15N] TFIIS-IWS1, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIS-IWS1	[U-13C; U-15N]		0.35 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

6 3D HCCH-TOCSY

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.35 mM [U-13C; U-15N] TFIIS-IWS1, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIS-IWS1	[U-13C; U-15N]		0.35 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

7 3D HBHA(CO)NH

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 0.35 mM [U-13C; U-15N] TFIIS-IWS1, 25 mM [U-2H] TRIS, 200 mM sodium chloride, 1 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TFIIS-IWS1	[U-13C; U-15N]		0.35 mM
2	TRIS	[U-2H]		25 mM
3	sodium chloride	natural abundance		200 mM
4	TCEP	natural abundance		1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34541_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34541_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1304		88.8 (chain: 1, length: 116)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 116, Sequence coverage: 88.8%
- Total number of assignments
  - ¹H chemical shifts: 749
  - ¹³C chemical shifts: 447
  - ¹⁵N chemical shifts: 108
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords TRANSCRIPTION, transcription elongation