BMRBj - BMREntryMaster

Found 1 Document (0.516 seconds)

BMRB: 34552

7A2D

Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation to the cell division site

Authors

Morris, F.C., Knowles, T.J., Maderbocus, R., Jeeves, M., Kirwan, J., Heinz, E., Wells, T.J., Browning, D.F., Sevastsyanovich, Y.R., Leyton, D.L., Rossiter, A.E., Bavro, V.N., Sridhar, P., Ward, D.G., Shimwell, N.J., Martin, A., Sahl, J.W., Wardius, C.A., Walker, D., Lithgow, T., Viant, M.R., Rasko, D.A., Cunningham, A.F., Overduin, M., Henderson, I.R.

Assembly

DolP

Entity

1. DolP (polymer, Thiol state: not present), 182 monomers, 19297.50 Da Detail

IAAAVVGTAA VGTKAATDPR SVGTQVDDGT LEVRVNSALS KDEQIKKEAR INVTAYQGKV LLVGQSPNAE LSARAKQIAM GVDGANEVYN EIRQGQPIGL GEASNDTWIT TKVRSQLLTS DLVKSSNVKV TTENGEVFLM GLVTEREAKA AADIASRVSG VKRVTTAFTF IKGGLEHHHH HH

Formula weight

19297.5 Da

Source organism

Escherichia coli KTE10

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 83.6 %, Completeness (bb): 94.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.6 % (1671 of 1999)	79.3 % (808 of 1019)	89.2 % (691 of 775)	83.9 % (172 of 205)
Backbone	94.3 % (1024 of 1086)	91.2 % (344 of 377)	95.8 % (508 of 530)	96.1 % (172 of 179)
Sidechain	74.7 % (806 of 1079)	72.1 % (463 of 642)	83.5 % (343 of 411)	0.0 % (0 of 26)
Aromatic	42.7 % (35 of 82)	43.9 % (18 of 41)	42.5 % (17 of 40)	0.0 % (0 of 1)
Methyl	91.8 % (224 of 244)	91.8 % (112 of 122)	91.8 % (112 of 122)

1. entity 1

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6 (±0.05), Details 1.5 mM [U-13C; U-15N] DolP, 50 mM NA sodium phosphate, 0.02 % w/v NA NaN3, 50 mM NA sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

Release date

2020-09-07

Citation

Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation
Bryant, J.A., Morris, F.C., Knowles, T.J., Maderbocus, R., Heinz, E., Boelter, G., Alodaini, D., Colyer, A., Wotherspoon, P.J., Staunton, K.A., Jeeves, M., Browning, D.F., Sevastsyanovich, Y.R., Wells, T.J., Rossiter, A.E., Bavro, V.N., Sridhar, P., Ward, D.G., Chong, Z.S., Goodall, E.C., Icke, C., Teo, A.C., Chng, S.S., Roper, D.I., Lithgow, T., Cunningham, A.F., Banzhaf, M., Overduin, M., Henderson, I.R.
Elife (2020), 9, PubMed 33315009 , DOI 10.7554/eLife.62614 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 19760 released on 2014-02-18
Title PlpA plays a central role in lipid homeostasis in Gram-negative bacterial outer membranes

Related entities 1. DolP, : 1 : 7 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6 (±0.05), Details 1.5 mM [U-13C; U-15N] DolP, 50 mM NA sodium phosphate, 0.02 % w/v NA NaN3, 50 mM NA sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

2 3D HN(CO)CA

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

3 3D HNCA

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

5 3D HNCACB

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

6 3D HNCO

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

7 3D HN(CA)CO

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

8 3D HCCH-TOCSY

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

9 3D 1H-13C NOESY-HSQC ALIPHATIC

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

10 3D 1H-13C NOESY-HSQC AROMATIC

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

11 3D 1H-15N NOESY-HSQC

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

12 H(C)CONH

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

13 3D HCCH-TOCSY

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DolP	[U-13C; U-15N]	1.5 mM
2	sodium phosphate	natural abundance	50 mM
3	NaN3	natural abundance	0.02 % w/v
4	sodium chloride	natural abundance	50 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34552_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34552_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1881		96.7 (chain: 1, length: 182)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 182, Sequence coverage: 96.7%
- Total number of assignments
  - ¹H chemical shifts: 1023
  - ¹³C chemical shifts: 686
  - ¹⁵N chemical shifts: 172
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1019	805	79.0
¹³C chemical shifts	775	686	88.5
¹⁵N chemical shifts	205	172	83.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	377	345	91.5
¹³C chemical shifts	364	347	95.3
¹⁵N chemical shifts	179	172	96.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	642	460	71.7
¹³C chemical shifts	411	339	82.5
¹⁵N chemical shifts	26	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	124	109	87.9
¹³C chemical shifts	124	109	87.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	41	18	43.9
¹³C chemical shifts	40	17	42.5
¹⁵N chemical shifts	1	0	0.0

Keywords GRAM NEGATIVE, LIPID BIOGENESIS, LIPOPROTEIN, OUTER MEMBRANE BIOGENESIS, PROTEIN BINDING, YRAP

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Found 1 Document (0.516 seconds)

BMRB: 34552

Sample

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. DolP, : 1 : 7 entities Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 3 contents Detail