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Found 1 Document (0.519 seconds)

BMRB: 19760

7A2D

PlpA plays a central role in lipid homeostasis in Gram-negative bacterial outer membranes

Authors

Morris, F.C., Knowles, T.J., Maderbocus, R., Jeeves, M., Kirwan, J., Heinz, E., Wells, T.J., Browning, D.F., Sevastsyanovich, Y.R., Leyton, D.L., Rossiter, A.E., Bavro, V.N., Sridhar, P., Ward, D.G., Shimwell, N.J., Martin, A., Sahl, J.W., Wardius, C.A., Walker, D., Lithgow, T., Viant, M.R., Rasko, D.A., Cunningham, A.F., Overduin, M., Henderson, I.R.

Assembly

PlpA

Entity

1. PlpA (polymer, Thiol state: not present), 182 monomers, 19297.50 Da Detail

IAAAVVGTAA VGTKAATDPR SVGTQVDDGT LEVRVNSALS KDEQIKKEAR INVTAYQGKV LLVGQSPNAE LSARAKQIAM GVDGANEVYN EIRQGQPIGL GEASNDTWIT TKVRSQLLTS DLVKSSNVKV TTENGEVFLM GLVTEREAKA AADIASRVSG VKRVTTAFTF IKGGLEHHHH HH

Formula weight

19297.5 Da

Source organism

Escherichia coli

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 78.5 %, Completeness (bb): 90.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.5 % (1569 of 1999)	71.7 % (731 of 1019)	85.8 % (665 of 775)	84.4 % (173 of 205)
Backbone	90.1 % (979 of 1086)	83.8 % (316 of 377)	92.5 % (490 of 530)	96.6 % (173 of 179)
Sidechain	69.1 % (746 of 1079)	64.6 % (415 of 642)	80.5 % (331 of 411)	0.0 % (0 of 26)
Aromatic	42.7 % (35 of 82)	43.9 % (18 of 41)	42.5 % (17 of 40)	0.0 % (0 of 1)
Methyl	87.3 % (213 of 244)	85.2 % (104 of 122)	89.3 % (109 of 122)

1. PlpA

IAAAVVGTAA VGTKAATDPR SVGTQVDDGT LEVRVNSALS KDEQIKKEAR INVTAYQGKV LLVGQSPNAE LSARAKQIAM GVDGANEVYN EIRQGQPIGL GEASNDTWIT TKVRSQLLTS DLVKSSNVKV TTENGEVFLM GLVTEREAKA AADIASRVSG VKRVTTAFTF IKGGLEHHHH HH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Release date

2014-02-18

Citation

Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation
Bryant, J.A., Morris, F.C., Knowles, T.J., Maderbocus, R., Heinz, E., Boelter, G., Alodaini, D., Colyer, A., Wotherspoon, P.J., Staunton, K.A., Jeeves, M., Browning, D.F., Sevastsyanovich, Y.R., Wells, T.J., Rossiter, A.E., Bavro, V.N., Sridhar, P., Ward, D.G., Chong, Z.S., Goodall, E.C., Icke, C., Teo, A.C., Chng, S.S., Roper, D.I., Lithgow, T., Cunningham, A.F., Banzhaf, M., Overduin, M., Henderson, I.R.
Elife (2020), 9, PubMed 33315009 , DOI 10.7554/eLife.62614 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 34552 released on 2020-09-07
Title Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation to the cell division site

Related entities 1. PlpA, : 1 : 7 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

2 3D HN(CO)CA

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

3 3D HNCA

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

5 3D HNCACB

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

6 3D HNCO

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

7 3D HN(CA)CO

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

8 3D H(C)CH-TOCSY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

9 3D (H)CCH-TOCSY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

10 3D 1H-13C NOESY-HSQC aliphatic

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

11 3D 1H-13C NOESY-HSQC aromatic

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

12 3D 1H-15N NOESY-HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

13 H(C)CONH

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	PlpA	[U-98% 13C; U-98% 15N]		1.5 mM
2	sodium phosphate	natural abundance		50 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

nullKeywords Gram negative, Outer membrane biogenesis, YraP, lipid biogenesis, lipoprotein