Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation to the cell division site
IAAAVVGTAA VGTKAATDPR SVGTQVDDGT LEVRVNSALS KDEQIKKEAR INVTAYQGKV LLVGQSPNAE LSARAKQIAM GVDGANEVYN EIRQGQPIGL GEASNDTWIT TKVRSQLLTS DLVKSSNVKV TTENGEVFLM GLVTEREAKA AADIASRVSG VKRVTTAFTF IKGGLEHHHH HH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.6 % (1671 of 1999) | 79.3 % (808 of 1019) | 89.2 % (691 of 775) | 83.9 % (172 of 205) |
Backbone | 94.3 % (1024 of 1086) | 91.2 % (344 of 377) | 95.8 % (508 of 530) | 96.1 % (172 of 179) |
Sidechain | 74.7 % (806 of 1079) | 72.1 % (463 of 642) | 83.5 % (343 of 411) | 0.0 % (0 of 26) |
Aromatic | 42.7 % (35 of 82) | 43.9 % (18 of 41) | 42.5 % (17 of 40) | 0.0 % (0 of 1) |
Methyl | 91.8 % (224 of 244) | 91.8 % (112 of 122) | 91.8 % (112 of 122) |
1. entity 1
IAAAVVGTAA VGTKAATDPR SVGTQVDDGT LEVRVNSALS KDEQIKKEAR INVTAYQGKV LLVGQSPNAE LSARAKQIAM GVDGANEVYN EIRQGQPIGL GEASNDTWIT TKVRSQLLTS DLVKSSNVKV TTENGEVFLM GLVTEREAKA AADIASRVSG VKRVTTAFTF IKGGLEHHHH HHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6 (±0.05), Details 1.5 mM [U-13C; U-15N] DolP, 50 mM NA sodium phosphate, 0.02 % w/v NA NaN3, 50 mM NA sodium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DolP | [U-13C; U-15N] | 1.5 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | NaN3 | natural abundance | 0.02 % w/v | |
4 | sodium chloride | natural abundance | 50 mM |