BMRB: 34600

N-terminal C2H2 Zn-finger domain of Clamp

Authors

Mariasina, S.S., Bonchuk, A.N., Tikhonova, E.A., Efimov, S.V., Maksimenko, O.G., Georgiev, P.G., Polshakov, V.I.

Assembly

Chromatin-linked adaptor for MSL proteins, isoform A

Entity

1. Chromatin-linked adaptor for MSL proteins, isoform A, entity 1 (polymer, Thiol state: all other bound), 70 monomers, 7404.340 Da Detail

SGSNGMPLNQ GAALGIATVD AQGRIQIVNQ NKPIAANTIS NISFKCDVCS DMFPHLALLN AHKRMHTDGE

2. Chromatin-linked adaptor for MSL proteins, isoform A, entity ZN (non-polymer), 65.409 Da

Total weight

7469.749 Da

Max. entity weight

7404.34 Da

Entity Connection

na 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS46:SG	2:ZN1:ZN
2	na	sing	1:CYS49:SG	2:ZN1:ZN
3	na	sing	1:HIS62:NE2	2:ZN1:ZN
4	na	sing	1:HIS66:NE2	2:ZN1:ZN

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 79.7 %, Completeness (bb): 94.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.7 % (612 of 768)	76.3 % (302 of 396)	83.2 % (243 of 292)	83.7 % (67 of 80)
Backbone	94.7 % (392 of 414)	91.6 % (131 of 143)	96.1 % (196 of 204)	97.0 % (65 of 67)
Sidechain	67.7 % (283 of 418)	68.0 % (172 of 253)	71.7 % (109 of 152)	15.4 % (2 of 13)
Aromatic	68.8 % (22 of 32)	93.8 % (15 of 16)	43.8 % (7 of 16)
Methyl	60.3 % (47 of 78)	64.1 % (25 of 39)	56.4 % (22 of 39)

1. entity 1

SGSNGMPLNQ GAALGIATVD AQGRIQIVNQ NKPIAANTIS NISFKCDVCS DMFPHLALLN AHKRMHTDGE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.3 mM [U-95% 15N] Clamp[86...153], 20 mM Na2HPO4/HaH2PO4, 50 mM NaCl, 1 mM DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Clamp[86...153]	[U-95% 15N]		0.3 mM
2	Na2HPO4/HaH2PO4	natural abundance		20 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		1 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.3 mM [U-99% 13C; U-99% 15N] Clamp[86...153], 20 mM Na2HPO4/HaH2PO4, 50 mM NaCl, 1 mM DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
5	Clamp[86...153]	[U-99% 13C; U-99% 15N]		0.3 mM
6	Na2HPO4/HaH2PO4	natural abundance		20 mM
7	NaCl	natural abundance		50 mM
8	DTT	natural abundance		1 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.3 mM [U-99% 13C; U-99% 15N] Clamp[86...153], 20 mM Na2HPO4/HaH2PO4, 50 mM NaCl, 1 mM DTT, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	Clamp[86...153]	[U-99% 13C; U-99% 15N]		0.3 mM
10	Na2HPO4/HaH2PO4	natural abundance		20 mM
11	NaCl	natural abundance		50 mM
12	DTT	natural abundance		1 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.3 mM [U-95% 15N] Clamp[86...153], 20 mM Na2HPO4/HaH2PO4, 50 mM NaCl, 1 mM DTT, 100% D2O

#	Name	Isotope labeling	Type	Concentration
13	Clamp[86...153]	[U-95% 15N]		0.3 mM
14	Na2HPO4/HaH2PO4	natural abundance		20 mM
15	NaCl	natural abundance		50 mM
16	DTT	natural abundance		1 mM

Hide sample codition

Release date

2021-02-08

Citation

Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila
Tikhonova, E.A., Mariasina, S.S., Efimov, S.V., Polshakov, V.I., Maksimenko, O.G., Georgiev, P.G., Bonchuk, A.N.
Nucleic Acids Res. (2022), 50, 6521-6531, PubMed 35648444 , DOI 10.1093/nar/gkac455 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Chromatin-linked adaptor for MSL proteins, isoform A, entity 1, : 1 : 1 : 6 entities Detail

Experiments performed 10 experiments Detail