BMRB: 4911

Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain

Authors

Fan, J., Zhang, Q., Tochio, H., Li, M., Zhang, M.

Assembly

DYNEIN light chain 8 (dlc8) and nNOS (fragment peptide) complex

Entity

1. DYNEIN light chain 8 (polymer, Thiol state: all free), 89 monomers, 10365.76 × 2 Da Detail

MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG

2. nNOS peptide (polymer, Thiol state: not present), 18 monomers, 2114.382 × 2 Da Detail

MKDTKIQVDR DLDGKSHK

Total weight

24960.283 Da

Max. entity weight

10365.76 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.2 %, Completeness: 69.2 %, Completeness (bb): 70.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	69.2 % (897 of 1296)	78.9 % (535 of 678)	53.0 % (266 of 502)	82.8 % (96 of 116)
Backbone	70.2 % (449 of 640)	82.1 % (179 of 218)	57.9 % (183 of 316)	82.1 % (87 of 106)
Sidechain	70.2 % (532 of 758)	77.4 % (356 of 460)	58.0 % (167 of 288)	90.0 % (9 of 10)
Aromatic	48.4 % (59 of 122)	95.1 % (58 of 61)	0.0 % (0 of 60)	100.0 % (1 of 1)
Methyl	85.0 % (85 of 100)	86.0 % (43 of 50)	84.0 % (42 of 50)

1. DYNEIN light chain 8

MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG

2. nNOS peptide

MKDTKIQVDR DLDGKSHK

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	DYNEIN light chain 8	[U-13C; U-15N]		1.5 mM
2	nNOS peptide	[U-13C; U-15N]		1.5 mM
3	phosphate buffer			100 mM
4	DTT			10 mM
5	D2O			10 %
6	H2O			90 %

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LACS Plot; CA

Referencing offset: -1.22 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -1.22 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Release date

2001-08-07

Citation

Structural basis of diverse sequence-dependent target recognition by the 8 kDa dynein light chain
Fan, J., Zhang, Q., Tochio, H., Li, M., Zhang, M.
J. Mol. Biol. (2001), 306, 97-108, PubMed 11178896 , DOI 10.1006/jmbi.2000.4374 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. DYNEIN light chain 8, : 3 : 1 : 115 entities Detail

Related entities 2. nNOS peptide, : 1 : 6 : 1 entities Detail

Interaction partners 2. nNOS peptide, : 9 interactors Detail

Experiments performed 2 experiments Detail