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Found 1 Document (4.978 seconds)

BMRB: 4912

1F3C

Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain

Authors

Fan, J., Zhang, Q., Tochio, H., Li, M., Zhang, M.

Assembly

DYNEIN

Entity

1. DYNEIN (polymer, Thiol state: all free), 89 monomers, 10365.76 × 2 Da Detail

MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG

Total weight

20731.52 Da

Max. entity weight

10365.76 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.6 %, Completeness (bb): 84.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.6 % (881 of 1080)	93.2 % (524 of 562)	62.2 % (262 of 421)	97.9 % (95 of 97)
Backbone	84.0 % (447 of 532)	98.3 % (178 of 181)	69.6 % (183 of 263)	97.7 % (86 of 88)
Sidechain	81.7 % (517 of 633)	90.8 % (346 of 381)	66.7 % (162 of 243)	100.0 % (9 of 9)
Aromatic	51.7 % (61 of 118)	96.6 % (57 of 59)	5.2 % (3 of 58)	100.0 % (1 of 1)
Methyl	97.7 % (84 of 86)	97.7 % (42 of 43)	97.7 % (42 of 43)

1. dynein light chain 8

MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG

Show sample condition

Sample

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	dynein light chain 8	[U-13C;U-15N]	1.5 mM
2	phosphate buffer		100 mM
3	DTT		10 mM
4	D2O		99.99 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -1.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -1.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1F3C, Strand ID: A, B Detail

Release date

2001-08-07

Citation

Structural basis of diverse sequence-dependent target recognition by the 8 kDa dynein light chain
Fan, J., Zhang, Q., Tochio, H., Li, M., Zhang, M.
J. Mol. Biol. (2001), 306, 97-108, PubMed 11178896 , DOI 10.1006/jmbi.2000.4374 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 4931 released on 2001-08-21
    Title Solution structure of dynein light chain 8 (DLC8) and bim peptide complex
  BMRB: 4911 released on 2001-08-07
    Title Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain
  Swiss-Prot: P63170 released on 2004-09-27
    Title DYL1_RAT Entity Dynein light chain 1, cytoplasmic

Related entities 1. DYNEIN, : 1 : 18 : 2 : 220 entities Detail

Interaction partners 1. DYNEIN, : 44 interactors Detail

More details for 44 interactors identified by 3 citations

Experiments performed 4 experiments Detail

1 2D NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	dynein light chain 8	[U-13C;U-15N]	1.5 mM
2	phosphate buffer		100 mM
3	DTT		10 mM
4	D2O		99.99 %

Sample #2

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	dynein light chain 8	[U-15N]	1.5 mM
6	phosphate buffer		100 mM
7	DTT		10 mM
8	D2O		10 %
9	H2O		90 %

Sample #3

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
10	dynein light chain 8	[U-15N; U-13C]	1.5 mM
11	phosphate buffer		100 mM
12	D2O		10 %
13	H2O		90 %

2 3D 15N-separated NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	dynein light chain 8	[U-13C;U-15N]	1.5 mM
2	phosphate buffer		100 mM
3	DTT		10 mM
4	D2O		99.99 %

Sample #2

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	dynein light chain 8	[U-15N]	1.5 mM
6	phosphate buffer		100 mM
7	DTT		10 mM
8	D2O		10 %
9	H2O		90 %

Sample #3

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
10	dynein light chain 8	[U-15N; U-13C]	1.5 mM
11	phosphate buffer		100 mM
12	D2O		10 %
13	H2O		90 %

3 3D 13C-separated NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	dynein light chain 8	[U-13C;U-15N]	1.5 mM
2	phosphate buffer		100 mM
3	DTT		10 mM
4	D2O		99.99 %

Sample #2

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	dynein light chain 8	[U-15N]	1.5 mM
6	phosphate buffer		100 mM
7	DTT		10 mM
8	D2O		10 %
9	H2O		90 %

Sample #3

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
10	dynein light chain 8	[U-15N; U-13C]	1.5 mM
11	phosphate buffer		100 mM
12	D2O		10 %
13	H2O		90 %

4 HNHA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	dynein light chain 8	[U-13C;U-15N]	1.5 mM
2	phosphate buffer		100 mM
3	DTT		10 mM
4	D2O		99.99 %

Sample #2

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	dynein light chain 8	[U-15N]	1.5 mM
6	phosphate buffer		100 mM
7	DTT		10 mM
8	D2O		10 %
9	H2O		90 %

Sample #3

Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
10	dynein light chain 8	[U-15N; U-13C]	1.5 mM
11	phosphate buffer		100 mM
12	D2O		10 %
13	H2O		90 %

nullKeywords dynein, light chain, DLC8, microtubules

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Found 1 Document (4.978 seconds)

BMRB: 4912

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. DYNEIN, : 1 : 18 : 2 : 220 entities Detail

Interaction partners 1. DYNEIN, : 44 interactors Detail

Experiments performed 4 experiments Detail

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