BMRB: 5394

Sculpting of the Eukaryotic Branch Site Recognition Motif by a Conserved Pseudouridine

Authors

Newby, M.I., Greenbaum, N.L.

Assembly

U2 snRNA - Intron duplex

Entity

1. U2 snRNA - intron duplex (polymer, Thiol state: not present), 9 monomers, 2975.747 Da Detail

GGUGXAGUA

2. U2 snRNA - intron duplex (polymer, Thiol state: not present), 10 monomers, 3167.896 Da Detail

UACUAACACC

Total weight

6143.643 Da

Max. entity weight

3167.896 Da

Entity Connection

covalent 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	covalent	sing	1:G4:03'	1:PSU5:P
2	covalent	sing	1:PSU5:03'	1:A6:P

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 42.1 %, Completeness: 25.0 %, Completeness (bb): 21.3 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	25.0 % (39 of 156)	25.0 % (39 of 156)
Suger, PO4	21.3 % (23 of 108)	21.3 % (23 of 108)
Nucleobase	33.3 % (16 of 48)	33.3 % (16 of 48)
Aromatic	40.0 % (16 of 40)	40.0 % (16 of 40)

1. U2 snRNA - intron duplex

GGUGXAGUA

2. U2 snRNA - intron duplex

UACUAACACC

Show sample condition

Sample

Pressure 1 atm, Temperature 278 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	U2 snRNA - intron duplex			1.0 mM
2	U2 snRNA - intron duplex			1.0 mM
3	sodium phosphate			10 mM
4	D2O			10 %
5	H2O			90 %

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 52.6 %, Completeness: 28.8 %, Completeness (bb): 24.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polyribonucleotide

	Total	1H
All	28.8 % (90 of 312)	28.8 % (90 of 312)
Suger, PO4	24.5 % (53 of 216)	24.5 % (53 of 216)
Nucleobase	38.5 % (37 of 96)	38.5 % (37 of 96)
Aromatic	46.3 % (37 of 80)	46.3 % (37 of 80)

1. U2 snRNA - intron duplex

GGUGXAGUA

2. U2 snRNA - intron duplex

UACUAACACC

Show sample condition

Sample

Pressure 1 atm, Temperature 278 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	U2 snRNA - intron duplex			1.0 mM
2	U2 snRNA - intron duplex			1.0 mM
3	sodium phosphate			10 mM
4	D2O			10 %
5	H2O			90 %

Hide sample codition

Release date

2002-06-12

Citation

Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine
Newby, M.I., Greenbaum, N.L.
Nat. Struct. Biol. (2002), 9, 958-965, PubMed 12426583 , DOI 10.1038/nsb873 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Experiments performed 3 experiments Detail