BMRBj - BMREntryMaster

Found 1 Document (2.474 seconds)

BMRB: 5465

1M12

NMR solution structure of human Saposin C

Authors

de Alba, E., Weiler, S., Tjandra, N.

Assembly

SAPOSIN C

Entity

1. SAPOSIN C (polymer, Thiol state: all disulfide bound), 84 monomers, 9415.822 Da Detail

SDVYCEVCEF LVKEVTKLID NNKTEKEILD AFDKMCSKLP KSLSEECQEV VDTYGSSILS ILLEEVSPEL VCSMLHLCSG LVPR

Formula weight

9415.822 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS78:SG
2	disulfide	sing	1:CYS8:SG	1:CYS72:SG
3	disulfide	sing	1:CYS36:SG	1:CYS47:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.5 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.5 % (946 of 970)	97.6 % (490 of 502)	97.4 % (374 of 384)	97.6 % (82 of 84)
Backbone	98.0 % (488 of 498)	98.2 % (164 of 167)	97.6 % (244 of 250)	98.8 % (80 of 81)
Sidechain	97.3 % (539 of 554)	97.3 % (326 of 335)	97.7 % (211 of 216)	66.7 % (2 of 3)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	96.3 % (104 of 108)	98.1 % (53 of 54)	94.4 % (51 of 54)

1. SAPOSIN C

SDVYCEVCEF LVKEVTKLID NNKTEKEILD AFDKMCSKLP KSLSEECQEV VDTYGSSILS ILLEEVSPEL VCSMLHLCSG LVPR

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	SAPOSIN C	[U-15N]	1 mM
2	NaCl		10.0 ~ 20.0 mM
3	H20		90 %
4	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	SAPOSIN C	[U-13C; U-15N]	1 mM
6	NaCl		10.0 ~ 20.0 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
9	SAPOSIN C	[U-13C; U-15N]	1 mM
10	NaCl		10.0 ~ 20.0 mM
11	D20		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	sodium acetate	na	null	null	1.0
¹H	TSP	na	null	null	1.0
¹⁵N	urea	na	null	null	1.0

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	sodium acetate	na	null	null	1.0
¹H	TSP	na	null	null	1.0
¹⁵N	urea	na	null	null	1.0

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	sodium acetate	na	null	null	1.0
¹H	TSP	na	null	null	1.0
¹⁵N	urea	na	null	null	1.0

Referencing offset: 0.02 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	sodium acetate	na	null	null	1.0
¹H	TSP	na	null	null	1.0
¹⁵N	urea	na	null	null	1.0

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1M12, Strand ID: A Detail

Release date

2002-07-15

Citation

Solution structure of human saposin C: pH-dependent interaction with phospholipid vesicles
de Alba, E., Weiler, S., Tjandra, N.
Biochemistry (2003), 42, 14729-14740, PubMed 14674747 , DOI 10.1021/bi0301338 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 6158 released on 2004-03-21
Title NMR solution structure of human Saposin C in the presence of detergent micelles

Related entities 1. SAPOSIN C, : 1 : 1 : 5 : 184 entities Detail

Interaction partners 1. SAPOSIN C, : 19 interactors Detail

More details for 19 interactors identified by 12 citations

Experiments performed 7 experiments Detail

1 3D 15N-separated NOESY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	SAPOSIN C	[U-15N]	1 mM
2	NaCl		10.0 ~ 20.0 mM
3	H20		90 %
4	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	SAPOSIN C	[U-13C; U-15N]	1 mM
6	NaCl		10.0 ~ 20.0 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
9	SAPOSIN C	[U-13C; U-15N]	1 mM
10	NaCl		10.0 ~ 20.0 mM
11	D20		100 %

2 4D 13C/15N-separated NOESY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	SAPOSIN C	[U-15N]	1 mM
2	NaCl		10.0 ~ 20.0 mM
3	H20		90 %
4	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	SAPOSIN C	[U-13C; U-15N]	1 mM
6	NaCl		10.0 ~ 20.0 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
9	SAPOSIN C	[U-13C; U-15N]	1 mM
10	NaCl		10.0 ~ 20.0 mM
11	D20		100 %

3 4D 13C-separated NOESY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	SAPOSIN C	[U-15N]	1 mM
2	NaCl		10.0 ~ 20.0 mM
3	H20		90 %
4	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	SAPOSIN C	[U-13C; U-15N]	1 mM
6	NaCl		10.0 ~ 20.0 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
9	SAPOSIN C	[U-13C; U-15N]	1 mM
10	NaCl		10.0 ~ 20.0 mM
11	D20		100 %

4 3D 13C-separated TOCSY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	SAPOSIN C	[U-15N]	1 mM
2	NaCl		10.0 ~ 20.0 mM
3	H20		90 %
4	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	SAPOSIN C	[U-13C; U-15N]	1 mM
6	NaCl		10.0 ~ 20.0 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
9	SAPOSIN C	[U-13C; U-15N]	1 mM
10	NaCl		10.0 ~ 20.0 mM
11	D20		100 %

5 CBCACONH

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	SAPOSIN C	[U-15N]	1 mM
2	NaCl		10.0 ~ 20.0 mM
3	H20		90 %
4	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	SAPOSIN C	[U-13C; U-15N]	1 mM
6	NaCl		10.0 ~ 20.0 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
9	SAPOSIN C	[U-13C; U-15N]	1 mM
10	NaCl		10.0 ~ 20.0 mM
11	D20		100 %

6 HNCACB

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	SAPOSIN C	[U-15N]	1 mM
2	NaCl		10.0 ~ 20.0 mM
3	H20		90 %
4	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	SAPOSIN C	[U-13C; U-15N]	1 mM
6	NaCl		10.0 ~ 20.0 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
9	SAPOSIN C	[U-13C; U-15N]	1 mM
10	NaCl		10.0 ~ 20.0 mM
11	D20		100 %

7 experiments for residual dipolar coupling measurements

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DMX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	SAPOSIN C	[U-15N]	1 mM
2	NaCl		10.0 ~ 20.0 mM
3	H20		90 %
4	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	SAPOSIN C	[U-13C; U-15N]	1 mM
6	NaCl		10.0 ~ 20.0 mM
7	H20		90 %
8	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
9	SAPOSIN C	[U-13C; U-15N]	1 mM
10	NaCl		10.0 ~ 20.0 mM
11	D20		100 %

nullKeywords alpha-helices, disulfide bridges

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.474 seconds)

BMRB: 5465

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. SAPOSIN C, : 1 : 1 : 5 : 184 entities Detail

Interaction partners 1. SAPOSIN C, : 19 interactors Detail

Experiments performed 7 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3