BMRB: 5556

PHD Fingers as Protein Scaffolds

Authors

Kwan, A.H.Y., Matthews, J.M., Mackay, J.P.

Assembly

PHD WSTF

Entity

1. PHD (polymer, Thiol state: not reported), 61 monomers, 6764.749 Da Detail

GPLGSDHHME FCRVCKDGGE LLCCDTCPSS YHIHCLRPAL YEVPDGEWQC PRCTCPALKG K

2. ZN (non-polymer), 65.409 Da

Total weight

6830.158 Da

Max. entity weight

6764.749 Da

Entity Connection

metal coordination 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:HIS32:ND1	2:ZN1:ZN
2	metal coordination	sing	1:CYS12:SG	2:ZN1:ZN
3	metal coordination	sing	1:CYS15:SG	2:ZN1:ZN
4	metal coordination	sing	1:CYS35:SG	2:ZN1:ZN
5	metal coordination	sing	1:CYS24:SG	2:ZN1:ZN
6	metal coordination	sing	1:CYS27:SG	2:ZN1:ZN
7	metal coordination	sing	1:CYS50:SG	2:ZN1:ZN
8	metal coordination	sing	1:CYS53:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.9 %, Completeness: 77.6 %, Completeness (bb): 82.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	77.6 % (273 of 352)	77.6 % (273 of 352)
Backbone	82.0 % (100 of 122)	82.0 % (100 of 122)
Sidechain	75.7 % (174 of 230)	75.7 % (174 of 230)
Aromatic	74.1 % (20 of 27)	74.1 % (20 of 27)
Methyl	90.9 % (20 of 22)	90.9 % (20 of 22)

1. PHD

GPLGSDHHME FCRVCKDGGE LLCCDTCPSS YHIHCLRPAL YEVPDGEWQC PRCTCPALKG K

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PHD			1 mM
2	DTT			1 mM
3	sodium phosphate buffer			10 mM
4	NaCl			150 mM
5	H2O			95 %
6	D2O			5 %

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Calculated from 20 models in PDB: 1MM3, Strand ID: A Detail

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Release date

2003-07-31

Citation

Engineering a protein scaffold from a PHD finger
Kwan, A.H.Y., Gell, D.A., Verger, A., Crossley, M., Matthews, J.M., Mackay, J.P.
Structure (2003), 11, 803-813, PubMed 12842043 , DOI 10.1016/s0969-2126(03)00122-9 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. PHD, : 1 : 148 entities Detail

Interaction partners 1. PHD, : 69 interactors Detail

Experiments performed 3 experiments Detail