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Found 1 Document (1.802 seconds)

BMRB: 6110

1UUC

solution structure of a chimeric LEKTI-domain

Authors

Tidow, H., Lauber, T., Roesch, P., Marx, U.C.

Assembly

mutant of LEKTI domain 1

Entity

1. mutant of LEKTI domain 1 (polymer, Thiol state: all disulfide bound), 55 monomers, 6397.461 Da Detail

KNEDQEMCHE FQAFMKNGKL FCPQDKKPIQ SLDGIMFINK CATCKMILEK EAKSQ

Formula weight

6397.461 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS8:SG	1:CYS44:SG
2	disulfide	sing	0:CYS22:SG	1:CYS41:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 74.5 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	74.5 % (318 of 427)	71.3 % (261 of 366)	93.4 % (57 of 61)
Backbone	98.8 % (161 of 163)	99.1 % (109 of 110)	98.1 % (52 of 53)
Sidechain	59.5 % (157 of 264)	59.4 % (152 of 256)	62.5 % (5 of 8)
Aromatic	50.0 % (11 of 22)	50.0 % (11 of 22)
Methyl	72.2 % (13 of 18)	72.2 % (13 of 18)

1. mutant of serine proteinase inhibitor LEKTI

KNEDQEMCHE FQAFMKNGKL FCPQDKKPIQ SLDGIMFINK CATCKMILEK EAKSQ

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mutant of serine proteinase inhibitor LEKTI			1.5 mM
2	potassium phosphate			10 mM

Protein Blocks Logo

Calculated from 30 models in PDB: 1UUC, Strand ID: A Detail

Coupling constant

15 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1) Detail

Release date

2004-09-27

Citation

The solution structure of a chimeric LEKTI domain reveals a chameleon sequence
Tidow, H., Lauber, T., Vitzithum, K., Sommerhoff, C.P., Roesch, P., Marx, U.C.
Biochemistry (2004), 43, 11238-11247, PubMed 15366933 , DOI 10.1021/bi0492399 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 6180 released on 2005-05-16
    Title Solution structure of the 15th domain of LEKTI
  BMRB: 6179 released on 2004-04-14
    Title Solution Structure of the structured part of the 15th domain of LEKTI
  Swiss-Prot: Q9NQ38 released on 2001-05-04
    Title ISK5_HUMAN Entity Serine protease inhibitor Kazal-type 5

Related entities 1. mutant of LEKTI domain 1, : 1 : 3 : 88 entities Detail

Experiments performed 8 experiments Detail

1 2D-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mutant of serine proteinase inhibitor LEKTI			1.5 mM
2	potassium phosphate			10 mM

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	mutant of serine proteinase inhibitor LEKTI	[U-100% 15N]		1.3 mM
4	potassium phosphate			10 mM

2 2D-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mutant of serine proteinase inhibitor LEKTI			1.5 mM
2	potassium phosphate			10 mM

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	mutant of serine proteinase inhibitor LEKTI	[U-100% 15N]		1.3 mM
4	potassium phosphate			10 mM

3 2D-NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mutant of serine proteinase inhibitor LEKTI			1.5 mM
2	potassium phosphate			10 mM

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	mutant of serine proteinase inhibitor LEKTI	[U-100% 15N]		1.3 mM
4	potassium phosphate			10 mM

4 1H,15N-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mutant of serine proteinase inhibitor LEKTI			1.5 mM
2	potassium phosphate			10 mM

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	mutant of serine proteinase inhibitor LEKTI	[U-100% 15N]		1.3 mM
4	potassium phosphate			10 mM

5 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mutant of serine proteinase inhibitor LEKTI			1.5 mM
2	potassium phosphate			10 mM

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	mutant of serine proteinase inhibitor LEKTI	[U-100% 15N]		1.3 mM
4	potassium phosphate			10 mM

6 3D-1H,15N-TOCSY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mutant of serine proteinase inhibitor LEKTI			1.5 mM
2	potassium phosphate			10 mM

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	mutant of serine proteinase inhibitor LEKTI	[U-100% 15N]		1.3 mM
4	potassium phosphate			10 mM

7 3D-1H,15N-NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mutant of serine proteinase inhibitor LEKTI			1.5 mM
2	potassium phosphate			10 mM

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	mutant of serine proteinase inhibitor LEKTI	[U-100% 15N]		1.3 mM
4	potassium phosphate			10 mM

8 3D-1H,15N,15N-HMQC-NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mutant of serine proteinase inhibitor LEKTI			1.5 mM
2	potassium phosphate			10 mM

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	mutant of serine proteinase inhibitor LEKTI	[U-100% 15N]		1.3 mM
4	potassium phosphate			10 mM

nullKeywords putative serine proteinase inhibitor, chameleon sequence, LEKTI, SPINK5

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Found 1 Document (1.802 seconds)

BMRB: 6110

Sample

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. mutant of LEKTI domain 1, : 1 : 3 : 88 entities Detail

Experiments performed 8 experiments Detail

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