BMRB: 6844

Solution Structure of Drosophila melanogaster SNF RBD2

Authors

Cui, G., Li, C., Jin, C., Xia, B.

Assembly

U1 small nuclear ribonucleoprotein A

Entity

1. U1 small nuclear ribonucleoprotein A (polymer, Thiol state: not present), 83 monomers, 9509.860 Da Detail

AQTEQPPNQI LFLTNLPEET NEMMLSMLFN QFPGFKEVRL VPNRHDIAFV EFTTELQSNA AKEALQGFKI TPTHAMKITF AKK

Formula weight

9509.86 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

simulated annealing molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.6 %, Completeness (bb): 96.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.6 % (924 of 1020)	93.6 % (500 of 534)	85.4 % (339 of 397)	95.5 % (85 of 89)
Backbone	96.1 % (467 of 486)	97.5 % (158 of 162)	95.5 % (236 of 247)	94.8 % (73 of 77)
Sidechain	87.3 % (537 of 615)	91.9 % (342 of 372)	79.2 % (183 of 231)	100.0 % (12 of 12)
Aromatic	27.3 % (24 of 88)	50.0 % (22 of 44)	4.5 % (2 of 44)
Methyl	97.8 % (88 of 90)	97.8 % (44 of 45)	97.8 % (44 of 45)

1. U1 small nuclear ribonucleoprotein A

AQTEQPPNQI LFLTNLPEET NEMMLSMLFN QFPGFKEVRL VPNRHDIAFV EFTTELQSNA AKEALQGFKI TPTHAMKITF AKK

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	U1 small nuclear ribonucleoprotein A	[U-15N; U-13C]		1 mM
2	phosphate buffer			50 mM
3	EDTA			1 mM
4	NaCl			50 mM
5	H2O			90 %
6	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.38 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.38 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.25 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2AYM, Strand ID: A Detail

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Release date

2009-10-19

Citation

Structure and novel functional mechanism of Drosophila SNF in sex-lethal splicing
Jicheng, H., Gaofeng, C., Congmin, L., Cong, L., Erchang, S., Luhua, L., Changwen, J., Jiwu, W., Bin, X.
PLoS One (2009), 4, e6890-e6890, PubMed 19727396 , DOI 10.1371/journal.pone.0006890 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. U1 small nuclear ribonucleoprotein A, : 1 : 3 : 229 entities Detail

Interaction partners 1. U1 small nuclear ribonucleoprotein A, : 2 interactors Detail

Experiments performed 2 experiments Detail