BMRB: 6865

1H, 13C, and 15N chemical shift assignments for P. falciparum TRAP-TSR domain

Authors

Tossavainen, H., Permi, P., Kilpelainen, I.

Assembly

TRAP-TSR domain

Entity

1. TRAP-TSR domain (polymer, Thiol state: all disulfide bound), 49 monomers, 5442.964 Da Detail

GSASCGVWDE WSPCSVTCGK GTRSRKREIL HEGCTSEIQE QCEEERCPP

Formula weight

5442.964 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS34:SG
2	disulfide	sing	1:CYS14:SG	1:CYS42:SG
3	disulfide	sing	1:CYS18:SG	1:CYS47:SG

Source organism

Plasmodium falciparum

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 89.4 %, Completeness (bb): 80.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.4 % (474 of 530)	98.2 % (275 of 280)	75.5 % (151 of 200)	96.0 % (48 of 50)
Backbone	80.9 % (233 of 288)	96.0 % (96 of 100)	65.5 % (93 of 142)	95.7 % (44 of 46)
Sidechain	99.7 % (285 of 286)	99.4 % (179 of 180)	100.0 % (102 of 102)	100.0 % (4 of 4)
Aromatic	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (12 of 12)	100.0 % (2 of 2)
Methyl	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (14 of 14)

1. TRAP-TSR domain

GSASCGVWDE WSPCSVTCGK GTRSRKREIL HEGCTSEIQE QCEEERCPP

Show sample condition

Sample

Temperature 283 (±1) K, pH 6.6 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	TRAP-TSR domain	[U-13C; U-15N]	protein	0.5 mM

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LACS Plot; CA

Referencing offset: 0.89 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	H2O	protons	4.92 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.89 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	H2O	protons	4.92 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	H2O	protons	4.92 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2BBX, Strand ID: A Detail

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Release date

2006-08-06

Citation

The layered fold of the TSR domain of P. falciparum TRAP contains a heparin binding site
Tossavainen, H., Pihlajamaa, T., Huttunen, T.K., Raulo, E., Rauvala, H., Permi, P., Kilpelainen, I.
Protein Sci. (2006), 15, 1760-1768, PubMed 16815922 , DOI 10.1110/ps.052068506 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. TRAP-TSR domain, : 1 : 146 entities Detail

Experiments performed 7 experiments Detail