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BMRB: 6914


6914
2ARF
Solution structure of the Wilson ATPase N-domain in the presence of ATP
Authors
Dmitriev, O., Tsivkovski, R., Abildgaard, F., Morgan, C.T., Markley, J.L., Lutsenko, S.
Assembly
WILSON DISEASE ATPASE
Entity
1. WILSON DISEASE ATPASE (polymer, Thiol state: all free), 165 monomers, 17291.75 Da Detail

AGHMVPRVMR VLLLGDVATL PLRKVLAVVG TAEASSEHPL GVAVTKYCKE ELGTETLGYC TDFQAVPGCG IGCKVSNVEG ILAHSERPLS APASHLNEAG SLPAEKDAVP QTFSVLIGNR EWLRRNGLTI SSDVSDAMTD HEMKGQTAIL VAIDGVLCGM IAIAD


Formula weight
17291.75 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
torsion angle dynamics, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.4 %, Completeness: 91.0 %, Completeness (bb): 99.3 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All91.0 % (1618 of 1778)88.5 % (803 of 907)92.2 % (651 of 706)99.4 % (164 of 165)
Backbone99.3 % (967 of 974)99.1 % (335 of 338)99.4 % (476 of 479)99.4 % (156 of 157)
Sidechain83.8 % (799 of 953)82.2 % (468 of 569)85.9 % (323 of 376)100.0 % (8 of 8)
Aromatic22.1 % (15 of 68)29.4 % (10 of 34)12.1 % (4 of 33)100.0 % (1 of 1)
Methyl96.9 % (221 of 228)99.1 % (113 of 114)94.7 % (108 of 114)

1. Wilson Disease ATPase N-domain

AGHMVPRVMR VLLLGDVATL PLRKVLAVVG TAEASSEHPL GVAVTKYCKE ELGTETLGYC TDFQAVPGCG IGCKVSNVEG ILAHSERPLS APASHLNEAG SLPAEKDAVP QTFSVLIGNR EWLRRNGLTI SSDVSDAMTD HEMKGQTAIL VAIDGVLCGM IAIAD

Show sample condition
Sample

Pressure 1 atm, Temperature 300 (±1) K, pH 6.0 (±0.2)


#NameIsotope labelingTypeConcentration
1Wilson Disease ATPase N-domain[U-99% 13C; U-95% 15N]0.75 mM
2sodium phosphate50 mM
3DTT5 mM
4NaN350 mM
5ATP5 mM
6H2O95 %
7D2O5 %

LACS Plot; CA
Referencing offset: -0.27 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.27 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.05 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.14 ppm, Outliers: 6 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 2ARF, Strand ID: A Detail

Release date
2006-04-25
Citation 1
NMR assignment of the Wilson disease associated protein N-domain
Dmitriev, O., Tsivkovski, R., Abildgaard, F., Lutsenko, S.
J. Biomol. NMR (2006), 36 Suppl 1, 61-61, PubMed 16868859 , DOI 10.1007/s10858-006-9046-5 ,
Show more 1 citaion
Citation 2
Solution structure of the N-domain of Wilson disease protein: distinct nucleotide-binding environment and effects of disease mutations
Dmitriev, O., Tsivkovski, R., Abildgaard, F., Morgan, C.T., Markley, J.L., Lutsenko, S.
Proc. Natl. Acad. Sci. U. S. A. (2006), 103, 5302-5307, PubMed 16567646 , DOI 10.1073/pnas.0507416103 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 16761 released on 2010-03-23
    Title Solution structue of the N-domain of Wilson disease protein: Distinct nucleotide-binding environment and effects of disease mutations
Related entities 1. WILSON DISEASE ATPASE, : 1 : 2 : 85 entities Detail
More details for 88 related entities
Interaction partners 1. WILSON DISEASE ATPASE, : 2 interactors Detail
More details for 2 interactors identified by 1 citations
Experiments performed 3 experiments Detail
nullKeywords ATP7B, ATPase, ATP binding, copper transport, nucleotide binding, P-TYPE ATPase, Wilson Disease