BMRB: 7326

Homonuclear NMR Assignment of the Pheromone En-1

Authors

Placzek, W.J., Etezady-Esfarjani, T., Herrmann, T., Peti, W., Wuthrich, K.

Assembly

En-1 monomer

Entity

1. En-1 monomer (polymer, Thiol state: all disulfide bound), 52 monomers, 5630.082 Da Detail

NPEDWFTPDT CAYGDSNTAW TTCTTPGQTC YTCCSSCFDV VGEQACQMSA QC

Formula weight

5630.082 Da

Entity Connection

disulfide 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS11:SG	1:CYS37:SG
2	disulfide	sing	1:CYS23:SG	1:CYS33:SG
3	disulfide	sing	1:CYS34:SG	1:CYS46:SG
4	disulfide	sing	1:CYS30:SG	1:CYS52:SG

Source organism

Euplotes nobilii

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 66.5 %, Completeness (bb): 58.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C
All	66.5 % (317 of 477)	94.4 % (254 of 269)	30.3 % (63 of 208)
Backbone	58.4 % (150 of 257)	98.1 % (102 of 104)	31.4 % (48 of 153)
Sidechain	65.1 % (175 of 269)	92.1 % (152 of 165)	22.1 % (23 of 104)
Aromatic	51.7 % (30 of 58)	100.0 % (30 of 30)	0.0 % (0 of 28)
Methyl	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)

1. En-1

NPEDWFTPDT CAYGDSNTAW TTCTTPGQTC YTCCSSCFDV VGEQACQMSA QC

Show sample condition

Sample

Temperature 298 K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	En-1	[U-13C]	protein	0.9 (±0.05) mM
2	Sodium Phosphate		buffer	20 (±1.0) mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2NSV, Strand ID: A Detail

---

Release date

2007-09-16

Citation

Cold-adapted signal proteins: NMR structures of pheromones from the Antarctic ciliate Euplotes nobilii
Placzek, W.J., Etezady-Esfarjani, T., Herrmann, T., Pedrini, B., Peti, W., Alimenti, C., Luporini, P., Wuthrich, K.
IUBMB Life (2007), 59, 578-585, PubMed 17701553 , DOI 10.1080/15216540701258165 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. En-1 monomer, : 1 : 2 : 4 entities Detail

Experiments performed 4 experiments Detail