Resonance assignments of the metal sensor CzrA in the apo-, Zn2- and DNA-bound (42 kDa) states
MAEQYSEINT DTLERVTEIF KALGDYNRIR IMELLSVSEA SVGHISHQLN LSQSNVSHQL KLLKSVHLVK AKRQGQSMIY SLDDIHVATM LKQAIHHANH PKESGL
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:ASP84:OD1 | 2:ZN1:ZN |
2 | na | sing | 1:ASP84:OD2 | 2:ZN1:ZN |
3 | na | sing | 1:HIS86:ND1 | 2:ZN1:ZN |
4 | na | sing | 1:HIS97:ND1 | 2:ZN1:ZN |
5 | na | sing | 1:HIS100:NE2 | 2:ZN1:ZN |
6 | na | sing | 1:ASP84:OD1 | 2:ZN1:ZN |
7 | na | sing | 1:ASP84:OD2 | 2:ZN1:ZN |
8 | na | sing | 1:HIS86:ND1 | 2:ZN1:ZN |
9 | na | sing | 1:HIS97:ND1 | 2:ZN1:ZN |
10 | na | sing | 1:HIS100:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 53.6 % (666 of 1243) | 31.0 % (200 of 645) | 73.6 % (354 of 481) | 95.7 % (112 of 117) |
Backbone | 80.9 % (513 of 634) | 51.6 % (111 of 215) | 95.2 % (299 of 314) | 98.1 % (103 of 105) |
Sidechain | 35.0 % (249 of 711) | 20.7 % (89 of 430) | 56.1 % (151 of 269) | 75.0 % (9 of 12) |
Aromatic | 6.1 % (4 of 66) | 12.1 % (4 of 33) | 0.0 % (0 of 33) | |
Methyl | 79.9 % (107 of 134) | 74.6 % (50 of 67) | 85.1 % (57 of 67) |
1. CzrA Chain
MAEQYSEINT DTLERVTEIF KALGDYNRIR IMELLSVSEA SVGHISHQLN LSQSNVSHQL KLLKSVHLVK AKRQGQSMIY SLDDIHVATM LKQAIHHANH PKESGLSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±2) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CzrA | [U-100% 13C; U-100% 15N] | 2 (±0.1) mM | |
2 | Zinc ion | natural abundance | 2.4 mM | |
3 | MES | [U-100% 2H] | 10 (±0.5) mM | |
4 | sodium chloride | natural abundance | 50 (±1.0) mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±2) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | CzrA | [U-2H; U-15N]; [U-13C]-Ile,Leu,Val-methyl | 2 (±0.1) mM | |
6 | Zinc ion | natural abundance | 2.4 mM | |
7 | MES | [U-100% 2H] | 10 (±0.5) mM | |
8 | sodium chloride | natural abundance | 50 (±1.0) mM |