BMRB: 15289

1H, 15N chemical shift assignment of the THAP domain 1-81 from human THAP1 protein

Authors

BESSIERE, D., CAMPAGNE, S., MILON, A., GERVAIS, V.

Assembly

THAP domain

Entity

1. THAP domain (polymer, Thiol state: free and other bound), 87 monomers, 10202.75 Da Detail

MVQSCSAYGC KNRYDKDKPV SFHKFPLTRP SLCKEWEAAV RRKNFKPTKY SSICSEHFTP DCFKRECNNK LLKENAVPTI FLELVPR

2. ZN (non-polymer), 65.409 Da

Total weight

10268.159 Da

Max. entity weight

10202.75 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	2:ZN1:ZN
2	disulfide	sing	1:CYS10:SG	2:ZN1:ZN
3	disulfide	sing	1:CYS54:SG	2:ZN1:ZN
4	disulfide	sing	1:HIS57:HE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.1 %, Completeness: 87.1 %, Completeness (bb): 89.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	87.1 % (572 of 657)	87.4 % (498 of 570)	85.1 % (74 of 87)
Backbone	89.5 % (222 of 248)	90.5 % (152 of 168)	87.5 % (70 of 80)
Sidechain	85.6 % (350 of 409)	86.1 % (346 of 402)	57.1 % (4 of 7)
Aromatic	98.1 % (52 of 53)	98.1 % (51 of 52)	100.0 % (1 of 1)
Methyl	85.3 % (29 of 34)	85.3 % (29 of 34)

1. THAP domain

MVQSCSAYGC KNRYDKDKPV SFHKFPLTRP SLCKEWEAAV RRKNFKPTKY SSICSEHFTP DCFKRECNNK LLKENAVPTI FLELVPR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	THAP domain	natural abundance		1 mM
2	NaCl	natural abundance		10 mM
3	Tris	natural abundance		50 mM
4	DTT	natural abundance		1 mM

Sample #2

Pressure 1 atm, Temperature 296 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
5	THAP domain	[U-15N]		1 mM
6	NaCl	natural abundance		10 mM
7	Tris	natural abundance		50 mM
8	DTT	natural abundance		1 mM

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Release date

2008-02-20

Citation

Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways
Bessiere, D., Lacroix, C., Campagne, S., Ecochard, V., Guillet, V., Mourey, L., Lopez, F., Czaplicki, J., Demange, P., Milon, A., Cirard, J., Gervais, V.
J. Biol. Chem. (2008), 283, 4352-4363, PubMed 18073205 , DOI 10.1074/jbc.M707537200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. THAP domain, : 1 : 5 : 70 entities Detail

Interaction partners 1. THAP domain, : 62 interactors Detail

Experiments performed 6 experiments Detail

Chemical shift validation 4 contents Detail