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BMRB: 15300

1H, 15N, 13C chemical shift assignment of the THAP domain 1-90 from human THAP1 protein

Authors

BESSIERE, D., CAMPAGNE, S., MILON, A., GERVAIS, V.

Assembly

THAP domain

Entity

1. THAP domain (polymer, Thiol state: free and other bound), 96 monomers, 11254.95 Da Detail

MVQSCSAYGC KNRYDKDKPV SFHKFPLTRP SLCKEWEAAV RRKNFKPTKY SSICSEHFTP DCFKRECNNK LLKENAVPTI FLCTEPHDKK LELVPR

2. ZN (non-polymer), 65.409 Da

Total weight

11320.359 Da

Max. entity weight

11254.95 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	2:ZN1:ZN
2	disulfide	sing	1:CYS10:SG	2:ZN1:ZN
3	disulfide	sing	1:CYS54:SG	2:ZN1:ZN
4	disulfide	sing	1:HIS57:HE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.2 %, Completeness: 60.7 %, Completeness (bb): 68.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	60.7 % (717 of 1182)	74.4 % (467 of 628)	41.4 % (190 of 459)	63.2 % (60 of 95)
Backbone	68.4 % (383 of 560)	77.8 % (144 of 185)	63.4 % (182 of 287)	64.8 % (57 of 88)
Sidechain	55.2 % (396 of 717)	72.9 % (323 of 443)	26.2 % (70 of 267)	42.9 % (3 of 7)
Aromatic	43.5 % (47 of 108)	85.2 % (46 of 54)	0.0 % (0 of 53)	100.0 % (1 of 1)
Methyl	41.9 % (31 of 74)	70.3 % (26 of 37)	13.5 % (5 of 37)

1. THAP domain

MVQSCSAYGC KNRYDKDKPV SFHKFPLTRP SLCKEWEAAV RRKNFKPTKY SSICSEHFTP DCFKRECNNK LLKENAVPTI FLCTEPHDKK LELVPR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	THAP domain	natural abundance	1 mM
2	NaCl	natural abundance	10 mM
3	Tris	natural abundance	50 mM
4	DTT	natural abundance	1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	THAP domain	[U-13C; U-15N]	1 mM
6	NaCl	natural abundance	10 mM
7	Tris	natural abundance	50 mM
8	DTT	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -2.37 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -2.37 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 2 Detail

Release date

2008-02-20

Citation

Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways
Bessiere, D., Lacroix, C., Campagne, S., Ecochard, V., Guillet, V., Mourey, L., Lopez, F., Czaplicki, J., Demange, P., Milon, A., Cirard, J., Gervais, V.
J. Biol. Chem. (2008), 283, 4352-4363, PubMed 18073205 , DOI 10.1074/jbc.M707537200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 15289 released on 2008-02-20
    Title 1H, 15N chemical shift assignment of the THAP domain 1-81 from human THAP1 protein
  Swiss-Prot: Q9NVV9 released on 2003-04-11
    Title THAP1_HUMAN Entity THAP domain-containing protein 1

Related entities 1. THAP domain, : 3 : 76 entities Detail

Interaction partners 1. THAP domain, : 62 interactors Detail

More details for 62 interactors identified by 7 citations