Chemical shift assignments of the F104-to-5-flurotryptophan mutant of cardiac troponin C
MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKSEEELSDL FRMWDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEFLEFMKGV E
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.5 % (1589 of 1858) | 79.5 % (771 of 970) | 92.2 % (662 of 718) | 91.8 % (156 of 170) |
Backbone | 96.8 % (931 of 962) | 95.5 % (317 of 332) | 97.7 % (460 of 471) | 96.9 % (154 of 159) |
Sidechain | 77.1 % (806 of 1045) | 71.2 % (454 of 638) | 88.4 % (350 of 396) | 18.2 % (2 of 11) |
Aromatic | 37.9 % (44 of 116) | 43.1 % (25 of 58) | 31.6 % (18 of 57) | 100.0 % (1 of 1) |
Methyl | 100.0 % (148 of 148) | 100.0 % (74 of 74) | 100.0 % (74 of 74) |
1. F104(FTR)
MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKSEEELSDL FRMWDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEFLEFMKGV ESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.5) K, pH 6.7 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | F104(FTR) | [U-100% 13C; U-100% 15N] | 1.0 (±0.2) mM | |
2 | Calcium | natural abundance | 10 (±1.0) mM | |
3 | potassium chloride | natural abundance | 100 (±10.0) mM |