NMR structure of the ubiquitin associated (UBA) domain of p62 (SQSTM1) in complex with ubiquitin. RDC refined
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 39.5 % (591 of 1495) | 37.9 % (296 of 782) | 41.4 % (239 of 577) | 41.2 % (56 of 136) |
Backbone | 42.1 % (319 of 758) | 41.6 % (109 of 262) | 42.6 % (159 of 373) | 41.5 % (51 of 123) |
Sidechain | 37.7 % (322 of 854) | 36.0 % (187 of 520) | 40.5 % (130 of 321) | 38.5 % (5 of 13) |
Aromatic | 56.8 % (42 of 74) | 56.8 % (21 of 37) | 55.6 % (20 of 36) | 100.0 % (1 of 1) |
Methyl | 35.1 % (52 of 148) | 35.1 % (26 of 74) | 35.1 % (26 of 74) |
1. P62 UBA
GSPPEADPRL IESLSQMLSM GFSDEGGWLT RLLQTKNYDI GAALDTIQYS KH2. ubiquitin
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-100% 15N] | 1 mM | |
2 | ubiquitin | 6 mM | ||
3 | potassium phosphate | natural abundance | 50 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | sodium azide | natural abundance | 0.04 % | |
6 | D2O | [U-100% 2H] | 10 % | |
7 | H2O | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | entity | [U-100% 13C; U-100% 15N] | 1 mM | |
9 | ubiquitin | 6 mM | ||
10 | potassium phosphate | natural abundance | 50 mM | |
11 | sodium chloride | natural abundance | 50 mM | |
12 | sodium azide | natural abundance | 0.04 % | |
13 | D2O | [U-100% 2H] | 10 % | |
14 | H2O | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 5% Polyacrylamide gel
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | entity | [U-100% 15N] | 1 mM | |
16 | ubiquitin | 6 mM | ||
17 | potassium phosphate | natural abundance | 50 mM | |
18 | sodium chloride | natural abundance | 50 mM | |
19 | sodium azide | natural abundance | 0.04 % | |
20 | D2O | [U-100% 2H] | 10 % | |
21 | H2O | 90 % |