NMR assignment of jerdostatin from Trimeresurus jerdonii, with deletion of two residues (N45 G46) from the C-terminal end
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS4:SG | 1:CYS13:SG |
2 | disulfide | sing | 1:CYS9:SG | 1:CYS32:SG |
3 | disulfide | sing | 1:CYS10:SG | 1:CYS37:SG |
4 | disulfide | sing | 1:CYS22:SG | 1:CYS39:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.5 % (387 of 458) | 97.5 % (232 of 238) | 64.0 % (114 of 178) | 97.6 % (41 of 42) |
Backbone | 80.5 % (206 of 256) | 98.9 % (87 of 88) | 62.5 % (80 of 128) | 97.5 % (39 of 40) |
Sidechain | 90.1 % (218 of 242) | 96.7 % (145 of 150) | 78.9 % (71 of 90) | 100.0 % (2 of 2) |
Aromatic | 50.0 % (16 of 32) | 93.8 % (15 of 16) | 0.0 % (0 of 15) | 100.0 % (1 of 1) |
Methyl | 100.0 % (24 of 24) | 100.0 % (12 of 12) | 100.0 % (12 of 12) |
1. jerdostatin -N45G46
AMDCTTGPCC RQCKLKPAGT TCWRTSVSSH YCTGRSCECP SYPGSolvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | jerdostatin -N45G46 | [U-100% 15N] | 0.5 mM | |
2 | D2O | natural abundance | 10 % | |
3 | H2O | natural abundance | 90 % |