NMR assignment of jerdostatin mutant R24K from Trimeresurus jerdonii, with deletion of two residues (N45 G46) from the end C-terminal
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS4:SG | 1:CYS13:SG |
2 | disulfide | sing | 1:CYS9:SG | 1:CYS32:SG |
3 | disulfide | sing | 1:CYS10:SG | 1:CYS37:SG |
4 | disulfide | sing | 1:CYS22:SG | 1:CYS39:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.1 % (396 of 460) | 98.7 % (236 of 239) | 66.5 % (119 of 179) | 97.6 % (41 of 42) |
Backbone | 81.6 % (209 of 256) | 98.9 % (87 of 88) | 64.8 % (83 of 128) | 97.5 % (39 of 40) |
Sidechain | 92.2 % (225 of 244) | 98.7 % (149 of 151) | 81.3 % (74 of 91) | 100.0 % (2 of 2) |
Aromatic | 53.1 % (17 of 32) | 100.0 % (16 of 16) | 0.0 % (0 of 15) | 100.0 % (1 of 1) |
Methyl | 100.0 % (24 of 24) | 100.0 % (12 of 12) | 100.0 % (12 of 12) |
1. jerdostatin R24K -N45G46
AMDCTTGPCC RQCKLKPAGT TCWKTSVSSH YCTGRSCECP SYPGSolvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 300 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | jerdostatin R24K -N45G46 | [U-100% 15N] | 0.5 mM |