Backbone and side chain 1H chemical shift assignments for acidic fibrobast growth factor bound to 1,3,6-naphthalenetrisulfonate
KKPKLLYCSN GGHFLRILPD GTVDGTRDRS DQHIQLQLSA ESVGEVYIKS TETGQYLAMD TDGLLYGSQT PNEECLFLER LEENHYNTYI SKKHAEKNWF VGLKKNGSCK RGPRTHYGQK AILFLPLPVS SD
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 81.7 % (666 of 815) | 81.7 % (666 of 815) |
Backbone | 94.4 % (255 of 270) | 94.4 % (255 of 270) |
Sidechain | 75.4 % (411 of 545) | 75.4 % (411 of 545) |
Aromatic | 90.6 % (58 of 64) | 90.6 % (58 of 64) |
Methyl | 93.8 % (60 of 64) | 93.8 % (60 of 64) |
1. aFGF
KKPKLLYCSN GGHFLRILPD GTVDGTRDRS DQHIQLQLSA ESVGEVYIKS TETGQYLAMD TDGLLYGSQT PNEECLFLER LEENHYNTYI SKKHAEKNWF VGLKKNGSCK RGPRTHYGQK AILFLPLPVS SDSolvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298 K, pH 6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | aFGF | natural abundance | 1.0 ~ 2.0 mM | |
2 | sodium phosphate | natural abundance | 10 mM | |
3 | TSP | natural abundance | 0.1 ~ 0.2 mM | |
4 | beta-mercaptoethanol | natural abundance | 1 mM | |
5 | sodium chloride | natural abundance | 150 mM | |
6 | NTS | natural abundance | 2 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1.0 atm, Temperature 298 K, pH 6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | aFGF | natural abundance | 1.0 ~ 2.0 mM | |
10 | sodium phosphate | natural abundance | 10 mM | |
11 | TSP | natural abundance | 0.1 ~ 0.2 mM | |
12 | beta-mercaptoethanol | natural abundance | 1 mM | |
13 | sodium chloride | natural abundance | 150 mM | |
14 | NTS | natural abundance | 2 mM | |
15 | D2O | natural abundance | 100 % |