BMRB: 17055

Binding Site Structure of One LRP-RAP Complex Implications for a Common Ligand-Receptor Binding Motif

Authors

Jensen, G.A., Andersen, O.M., Bonvin, A.M.J.J., Bjerrum-Bohr, I., Etzerodt, M., Thogersen, H.C., O'Shea, C., Poulsen, F.M., Kragelund, B.B.

Assembly

CR56/RAPd1 complex

Entity

1. CR56 (polymer, Thiol state: not reported), 82 monomers, 8938.567 Da Detail

SARTCPPNQF SCASGRCIPI SWTCDLDDDC GDRSDESASC AYPTCFPLTQ FTCNNGRCIN INWRCDNDND CGDNSDEAGC SH

2. RAPd1 (polymer, Thiol state: not present), 81 monomers, 9516.852 Da Detail

GEEFRMEKLN QLWEKAQRLH LPPVRLAELH ADLKIQERDE LAWKKLKLDG LDEDGEKEAR LIRNLNVILA KYGLDGKKDA R

Total weight

18455.418 Da

Max. entity weight

9516.852 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

binding_constants

Binding constant

1 Kd values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 7.0 Detail

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List #1 binding_data, Participants 1. CR56, 2. RAPd1,

Sample ID	Exp. name	Binding reaction	Obs. entity ID	Obs. parameter	Value type	Value	Value error
1	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	2 uM

Release date

2010-09-07

Citation

Binding site structure of one LRP-RAP complex: implications for a common ligand-receptor binding motif
Jensen, G.A., Andersen, O.M., Bonvin, A.M.J.J., Bjerrum-Bohr, I., Etzerodt, M., Thogersen, H.C., O'Shea, C., Poulsen, F.M., Kragelund, B.
J. Mol. Biol. (2006), 362, 700-716, PubMed 16938309 , DOI 10.1016/j.jmb.2006.07.013 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

Related entities 1. CR56, : 2 : 1 : 748 entities Detail

Related entities 2. RAPd1, : 1 : 8 : 10 entities Detail

Interaction partners 1. CR56, : 22 : 1 interactors Detail

Interaction partners 2. RAPd1, : 14 : 1 interactors Detail

Experiments performed 1 experiments Detail