Binding site structure of one LRP/RAP complex - implications for a common ligand/receptor binding motif
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS5:SG | 1:CYS17:SG |
2 | disulfide | sing | 1:CYS12:SG | 1:CYS30:SG |
3 | disulfide | sing | 1:CYS24:SG | 1:CYS40:SG |
4 | disulfide | sing | 1:CYS45:SG | 1:CYS58:SG |
5 | disulfide | sing | 1:CYS53:SG | 1:CYS71:SG |
6 | disulfide | sing | 1:CYS65:SG | 1:CYS80:SG |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 89.9 % (473 of 526) | 89.7 % (392 of 437) | 91.0 % (81 of 89) |
Backbone | 92.1 % (222 of 241) | 93.3 % (153 of 164) | 89.6 % (69 of 77) |
Sidechain | 88.1 % (251 of 285) | 87.5 % (239 of 273) | 100.0 % (12 of 12) |
Aromatic | 74.3 % (26 of 35) | 72.7 % (24 of 33) | 100.0 % (2 of 2) |
Methyl | 100.0 % (22 of 22) | 100.0 % (22 of 22) |
1. complement type repeats 5 and 6
SARTCPPNQF SCASGRCIPI SWTCDLDDDC GDRSDESASC AYPTCFPLTQ FTCNNGRCIN INWRCDNDND CGDNSDEAGC SHTemperature 298 (±1) K, pH 7.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | complement type repeats 5 and 6 | 0.5 mM | ||
2 | CaCl2 | 10 mM | ||
3 | MgCl2 | 1 mM | ||
4 | NaCl | 100 mM |
Temperature 298 (±1) K, pH 7.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | complement type repeats 5 and 6 | [U-99% 15N] | 0.5 mM | |
6 | CaCl2 | 10 mM | ||
7 | MgCl2 | 1 mM | ||
8 | NaCl | 100 mM |