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BMRB: 17063

4A5T

1H and 15N Chemical Shift Assignments of Mutated (K57D) Human Plasminogen Kringle 3

Authors

Christen, M.T., Frank, P., Schaller, J., Llinas, M.

Assembly

hPgn (K57D)rK3

Entity

1. hPgn (K57D)rK3 (polymer, Thiol state: all disulfide bound), 83 monomers, 9498.226 Da Detail

TYQCLKGTGE NYRGNVAVTV SGHTCQHWSA QTPHTHNRTP ENFPSKNLDE NYCRNPDGDR APWCHTTNSQ VRWEYCKIPS CDS

Formula weight

9498.226 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 97.6 %, Completeness: 72.2 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	72.2 % (418 of 579)	67.8 % (330 of 487)	95.7 % (88 of 92)
Backbone	93.8 % (227 of 242)	92.1 % (152 of 165)	97.4 % (75 of 77)
Sidechain	56.7 % (191 of 337)	55.3 % (178 of 322)	86.7 % (13 of 15)
Aromatic	36.5 % (19 of 52)	32.7 % (16 of 49)	100.0 % (3 of 3)
Methyl	80.8 % (21 of 26)	80.8 % (21 of 26)

1. Plasminogen Kringle 3 Domain (Mutated)

TYQCLKGTGE NYRGNVAVTV SGHTCQHWSA QTPHTHNRTP ENFPSKNLDE NYCRNPDGDR APWCHTTNSQ VRWEYCKIPS CDS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, Details 0.02% sodium azide was added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	hPgn (K57D)rK3	[U-98% 15N]	1.0 mM
2	H2O	natural abundance	90 %
3	H2O	natural abundance	10 %
4	sodium azide	natural abundance	0.02 %

Chem. Shift Complete2

Sequence coverage: 88.0 %, Completeness: 53.0 %, Completeness (bb): 77.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	53.0 % (614 of 1158)	45.6 % (444 of 974)	92.4 % (170 of 184)
Backbone	77.3 % (374 of 484)	69.1 % (228 of 330)	94.8 % (146 of 154)
Sidechain	35.6 % (240 of 674)	33.5 % (216 of 644)	80.0 % (24 of 30)
Aromatic	24.0 % (25 of 104)	19.4 % (19 of 98)	100.0 % (6 of 6)
Methyl	48.1 % (25 of 52)	48.1 % (25 of 52)

1. Plasminogen Kringle 3 Domain (Mutated)

TYQCLKGTGE NYRGNVAVTV SGHTCQHWSA QTPHTHNRTP ENFPSKNLDE NYCRNPDGDR APWCHTTNSQ VRWEYCKIPS CDS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, Details 0.02% sodium azide was added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	hPgn (K57D)rK3	[U-98% 15N]	1.0 mM
2	H2O	natural abundance	90 %
3	H2O	natural abundance	10 %
4	sodium azide	natural abundance	0.02 %

Release date

2010-08-18

Citation

Human plasminogen kringle 3: solution structure, functional insights, phylogenetic landscape
Christen, M.T., Frank, P., Schaller, J., Llinas, M.
Biochemistry (2010), 49, 7131-7150, PubMed 20617841 , DOI 10.1021/bi100687f , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17062 released on 2010-08-18
Title Solution Structure of Human Plasminogen Kringle 3

Related entities 1. hPgn (K57D)rK3, : 5 : 6 : 445 entities Detail

Interaction partners 1. hPgn (K57D)rK3, : 35 interactors Detail

More details for 35 interactors identified by 14 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC