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BMRB: 17300

6ZOM

1H, 13C and 15N Chemical shift assignments of the thioredoxin from the anaerobic bacteria Desulfovibrio vulgaris Hildenborough

Authors

Garcin, E.B., Bornet, O., Sebban-Kreuzer, C., Guerlesquin, F.

Assembly

thioredoxin

Entity

1. thioredoxin (polymer, Thiol state: all free), 113 monomers, 12259.08 Da Detail

HHHHHHMAAQ ITDATFEASV LKSAIPVLID FWAPWCGPCR AMGPVIDELA AEYEGKVLIV KMNVDDNPAT PSKYGIRAIP TLILFKNGEV VEQVTGAVSK SSIKDMIAQK ALG

Formula weight

12259.08 Da

Source organism

Desulfovibrio vulgaris

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 91.0 %, Completeness (bb): 91.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.0 % (1170 of 1286)	92.4 % (610 of 660)	88.5 % (453 of 512)	93.9 % (107 of 114)
Backbone	91.6 % (608 of 664)	92.0 % (208 of 226)	90.7 % (301 of 332)	93.4 % (99 of 106)
Sidechain	89.3 % (650 of 728)	90.3 % (392 of 434)	87.4 % (250 of 286)	100.0 % (8 of 8)
Aromatic	59.6 % (56 of 94)	70.2 % (33 of 47)	46.7 % (21 of 45)	100.0 % (2 of 2)
Methyl	98.6 % (144 of 146)	97.3 % (71 of 73)	100.0 % (73 of 73)

1. Thioredoxin

HHHHHHMAAQ ITDATFEASV LKSAIPVLID FWAPWCGPCR AMGPVIDELA AEYEGKVLIV KMNVDDNPAT PSKYGIRAIP TLILFKNGEV VEQVTGAVSK SSIKDMIAQK ALG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	reduced thioredoxin	[U-99% 13C; U-99% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	Potassium phosphate	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 1 Detail

Release date

2011-02-03

Citation

1H, 13C and 15N chemical shift assignments of the thioredoxin from the obligate anaerobe Desulfovibrio vulgaris Hildenborough
Garcin, E.B., Bornet, O., Pieulle, L., Guerlesquin, F., Sebban-Kreuzer, C.
Biomol. NMR Assign. (2011), 5, 177-179, PubMed 21287302 , DOI 10.1007/s12104-011-9294-5 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17299 released on 2011-02-03
Title 1H, 13C and 15N Chemical shift assignments of the thioredoxin from the anaerobic bacteria Desulfovibrio vulgaris Hildenborough

Related entities 1. thioredoxin, : 1 : 242 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC