BMRB: 17483

Structural characterization of small heat shock protein (Hsp12) in aqueous solution

Authors

Singarapu, K.K., Tonelli, M., Westler, W.M.

Assembly

hsp12

Entity

1. hsp12 (polymer, Thiol state: not present), 109 monomers, 11692.56 Da Detail

MSDAGRKGFG EKASEALKPD SQKSYAEQGK EYITDKADKV AGKVQPEDNK GVFQGVHDSA EKGKDNAEGQ GESLADQARD YMGAAKSKLN DAVEYVSGRV HGEEDPTKK

Formula weight

11692.56 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 67.9 %, Completeness (bb): 94.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	67.9 % (812 of 1196)	62.9 % (397 of 631)	69.6 % (313 of 450)	88.7 % (102 of 115)
Backbone	94.1 % (610 of 648)	93.9 % (214 of 228)	93.9 % (295 of 314)	95.3 % (101 of 106)
Sidechain	45.3 % (292 of 644)	45.4 % (183 of 403)	46.6 % (108 of 232)	11.1 % (1 of 9)
Aromatic	0.0 % (0 of 60)	0.0 % (0 of 30)	0.0 % (0 of 30)
Methyl	45.9 % (34 of 74)	45.9 % (17 of 37)	45.9 % (17 of 37)

1. hsp12

MSDAGRKGFG EKASEALKPD SQKSYAEQGK EYITDKADKV AGKVQPEDNK GVFQGVHDSA EKGKDNAEGQ GESLADQARD YMGAAKSKLN DAVEYVSGRV HGEEDPTKK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hsp12	[U-100% 15N]		0.2 mM
2	hsp12	[U-100% 13C; U-100% 15N]		1.0 mM

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LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.14 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Release date

2011-10-25

Citation

Structural characterization of Hsp12, the heat shock protein from Saccharomyces cerevisiae, in aqueous solution where it is intrinsically disordered and in detergent micelles where it is locally α-helical
Singarapu, K.K., Tonelli, M., Chow, D.C., Frederick, R.O., Westler, W.M., Markley, J.L.
J. Biol. Chem. (2011), 286, 43447-43453, PubMed 21998307 , DOI 10.1074/jbc.M111.306464 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. hsp12, : 1 : 4 : 3 entities Detail

Interaction partners 1. hsp12, : 104 interactors Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail