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BMRB: 17630

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 23-117

Authors

Burge, R.G., Martinez-Yamout, M.A., Gottesfeld, J.M., Dyson, H.J., Wright, P.E., Dyson, J.H.

Assembly

JAZ ZF1

Entity

1. JAZ ZF1 (polymer, Thiol state: free and other bound), 95 monomers, 10883.31 Da Detail

SELLEGQEPD GVRFDRERAR RLWEAVSGAQ PVGREEVEHM IQKNQCLFTN TQCKVCCALL ISESQKLAHY QSKKHANKVK RYLAIHGMET LKGET

2. ZN (non-polymer, Thiol state: not present), 65.409 Da

Total weight

10948.719 Da

Max. entity weight

10883.31 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.0 %, Completeness: 19.9 %, Completeness (bb): 38.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	19.9 % (223 of 1122)	13.0 % (77 of 592)	18.8 % (80 of 426)	63.5 % (66 of 104)
Backbone	38.7 % (219 of 566)	37.6 % (73 of 194)	28.7 % (80 of 279)	71.0 % (66 of 93)
Sidechain	1.2 % (8 of 645)	1.0 % (4 of 398)	1.7 % (4 of 236)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 64)	0.0 % (0 of 32)	0.0 % (0 of 31)	0.0 % (0 of 1)
Methyl	2.1 % (2 of 94)	4.3 % (2 of 47)	0.0 % (0 of 47)

1. JAZ ZF1

SELLEGQEPD GVRFDRERAR RLWEAVSGAQ PVGREEVEHM IQKNQCLFTN TQCKVCCALL ISESQKLAHY QSKKHANKVK RYLAIHGMET LKGET

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Concentration
1	JAZ ZF1	[U-99% 13C; U-99% 15N]	250.0 ~ 300.0 uM
2	TRIS	natural abundance	25 mM
3	potassium chloride	natural abundance	50 mM
4	D2O	[U-99% 2H]	7 %
5	DTT	natural abundance	2 mM
6	zinc	natural abundance	50 uM
7	H2O	natural abundance	93 %

Release date

2014-02-23

Citation

Structural characterization of interactions between the double-stranded RNA-binding zinc finger protein JAZ and nucleic acids
Burge, R.G., Martinez-Yamout, M.A., Dyson, H., Wright, P.E.
Biochemistry (2014), 53, 1495-1510, PubMed 24521053 , DOI 10.1021/bi401675h , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 17672 released on 2014-02-23
    Title Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 228-277
  BMRB: 17679 released on 2014-02-23
    Title Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 168-227
  Swiss-Prot: Q9UL40 released on 2005-10-25
    Title ZN346_HUMAN Entity Zinc finger protein 346

Related entities 1. JAZ ZF1, : 3 : 4 entities Detail

Interaction partners 1. JAZ ZF1, : 16 interactors Detail

More details for 16 interactors identified by 8 citations

Experiments performed 5 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr17630_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr17630_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	201		76.8 (chain: 1, length: 95)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 95, Sequence coverage: 76.8%
- Total number of assignments
  - ¹³C chemical shifts: 72
  - ¹H chemical shifts: 65
  - ¹⁵N chemical shifts: 64
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	426	72	16.9
¹H chemical shifts	592	65	11.0
¹⁵N chemical shifts	111	64	57.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	190	72	37.9
¹H chemical shifts	194	65	33.5
¹⁵N chemical shifts	93	64	68.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	236	0	0.0
¹H chemical shifts	398	0	0.0
¹⁵N chemical shifts	18	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	49	0	0.0
¹H chemical shifts	49	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	31	0	0.0
¹H chemical shifts	32	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords dsRNA, Just Another Zinc finger, RNA-protein interactions, Zinc Finger protein a

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Found 1 Document (0.96 seconds)

BMRB: 17630

Sample

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. JAZ ZF1, : 3 : 4 entities Detail

Interaction partners 1. JAZ ZF1, : 16 interactors Detail

Experiments performed 5 experiments Detail

Instrument

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Chemical shift validation 4 contents Detail