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Found 1 Document (6.209 seconds)

BMRB: 17672

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 228-277

Authors

Burge, R.G., Martinez-Yamout, M.A., Dyson, H.JANE., Wright, P.E.

Assembly

JAZ ZF4

Entity

1. JAZ ZF4 (polymer, Thiol state: all other bound), 50 monomers, 5362.056 Da Detail

PAVTDFPAGK GYPCKTCKIV LNSIEQYQAH VSGFKHKNQS PKTVASSLGQ

2. ZN (non-polymer), 65.409 Da

Total weight

5427.4653 Da

Max. entity weight

5362.056 Da

Entity Connection

na 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS14:SG	2:ZN1:ZN
2	na	sing	1:CYS17:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 66.0 %, Completeness: 17.4 %, Completeness (bb): 33.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	17.4 % (100 of 574)	11.3 % (34 of 300)	15.3 % (34 of 222)	61.5 % (32 of 52)
Backbone	33.2 % (97 of 292)	33.0 % (33 of 100)	22.6 % (33 of 146)	67.4 % (31 of 46)
Sidechain	0.6 % (2 of 328)	0.5 % (1 of 200)	0.8 % (1 of 122)	0.0 % (0 of 6)
Aromatic	0.0 % (0 of 44)	0.0 % (0 of 22)	0.0 % (0 of 22)
Methyl	0.0 % (0 of 46)	0.0 % (0 of 23)	0.0 % (0 of 23)

1. JAZ ZF4

PAVTDFPAGK GYPCKTCKIV LNSIEQYQAH VSGFKHKNQS PKTVASSLGQ

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	JAZ ZF4	[U-98% 13C; U-98% 15N]	0.4 (±0.04) mM
2	H2O	natural abundance	93 %
3	D2O	natural abundance	7 %
4	KCl	natural abundance	50 mM
5	Tris	natural abundance	25 mM
6	DTT	natural abundance	2 mM
7	ZnSO4	natural abundance	50 uM

Release date

2014-02-23

Citation

Structural characterization of interactions between the double-stranded RNA-binding zinc finger protein JAZ and nucleic acids
Burge, R.G., Martinez-Yamout, M.A., Dyson, H., Wright, P.E.
Biochemistry (2014), 53, 1495-1510, PubMed 24521053 , DOI 10.1021/bi401675h , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 17630 released on 2014-02-23
    Title Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 23-117
  BMRB: 17679 released on 2014-02-23
    Title Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 168-227
  Swiss-Prot: Q9UL40 released on 2005-10-25
    Title ZN346_HUMAN Entity Zinc finger protein 346

Related entities 1. JAZ ZF4, : 3 : 20 entities Detail

Interaction partners 1. JAZ ZF4, : 16 interactors Detail

More details for 16 interactors identified by 8 citations

Experiments performed 5 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr17672_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:14:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:17:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a

Non-standard residues

None

Content subtype: bmr17672_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	94		64.0 (chain: 1, length: 50)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 50, Sequence coverage: 64.0%
- Total number of assignments
  - ¹³C chemical shifts: 32
  - ¹H chemical shifts: 31
  - ¹⁵N chemical shifts: 31
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	222	32	14.4
¹H chemical shifts	300	31	10.3
¹⁵N chemical shifts	52	31	59.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	100	32	32.0
¹H chemical shifts	100	31	31.0
¹⁵N chemical shifts	46	31	67.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	122	0	0.0
¹H chemical shifts	200	0	0.0
¹⁵N chemical shifts	6	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	23	0	0.0
¹H chemical shifts	23	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	22	0	0.0
¹H chemical shifts	22	0	0.0

Keywords Just Another Zinc finger

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Found 1 Document (6.209 seconds)

BMRB: 17672

Sample

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. JAZ ZF4, : 3 : 20 entities Detail

Interaction partners 1. JAZ ZF4, : 16 interactors Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

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Instrument

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Chemical shift validation 4 contents Detail