NMR structure of holo-ACPI domain from CurA module from Lyngbya majuscula
SGLVPRGSHM TPQVNQVNLS EIKQVLKQQL AEALYTEESE IAEDQKFVDL GLDSIVGVEW TTTINQTYNL NLKATKLYDY PTLLELSGYI AQILSSQGT
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.1 % (1085 of 1153) | 93.6 % (556 of 594) | 93.8 % (420 of 448) | 98.2 % (109 of 111) |
Backbone | 96.6 % (568 of 588) | 96.0 % (193 of 201) | 96.2 % (280 of 291) | 99.0 % (95 of 96) |
Sidechain | 92.4 % (608 of 658) | 92.4 % (363 of 393) | 92.4 % (231 of 250) | 93.3 % (14 of 15) |
Aromatic | 86.4 % (57 of 66) | 90.9 % (30 of 33) | 84.4 % (27 of 32) | 0.0 % (0 of 1) |
Methyl | 86.8 % (118 of 136) | 89.7 % (61 of 68) | 83.8 % (57 of 68) |
1. entity
SGLVPRGSHM TPQVNQVNLS EIKQVLKQQL AEALYTEESE IAEDQKFVDL GLDSIVGVEW TTTINQTYNL NLKATKLYDY PTLLELSGYI AQILSSQGTSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 291 K, pH 6.8, Details 1mM Holo ACPI in 50mM Arg?Glu pH=6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CurA | [U-15N] | 1 mM | |
2 | D2O | natural abundance | 5 % | |
3 | DSS | natural abundance | 0.15 mM | |
4 | Arginine | natural abundance | 50 mM | |
5 | Glutamate | natural abundance | 50 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 291 K, pH 6.8, Details 1mM Holo ACPI in 50mM Arg/Glu pH=6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | CurA | [U-13C] | 1 mM | |
9 | D2O | natural abundance | 5 % | |
10 | DSS | natural abundance | 0.15 mM | |
11 | Arginine | natural abundance | 50 mM | |
12 | Glutamate | natural abundance | 50 mM | |
13 | H2O | natural abundance | 95 % | |
14 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 291 K, pH 6.8, Details 1mM Holo ACPI in 50mM Arg/Glu pH=6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | CurA | [U-15N],[U-13C] | 1 mM | |
16 | D2O | natural abundance | 5 % | |
17 | DSS | natural abundance | 0.15 mM | |
18 | Arginine | natural abundance | 50 mM | |
19 | Glutamate | natural abundance | 50 mM | |
20 | H2O | natural abundance | 95 % | |
21 | D2O | natural abundance | 5 % |