NMR structure of HMG-ACPI domain from CurA module from Lyngbya majuscula
SGLVPRGSHM TPQVNQVNLS EIKQVLKQQL AEALYTEESE IAEDQKFVDL GLDXIVGVEW TTTINQTYNL NLKATKLYDY PTLLELSGYI AQILSSQGT
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.4 % (1070 of 1145) | 92.5 % (546 of 590) | 93.5 % (416 of 445) | 98.2 % (108 of 110) |
Backbone | 95.9 % (558 of 582) | 95.5 % (190 of 199) | 95.5 % (275 of 288) | 97.9 % (93 of 95) |
Sidechain | 91.8 % (601 of 655) | 91.0 % (356 of 391) | 92.4 % (230 of 249) | 100.0 % (15 of 15) |
Aromatic | 89.4 % (59 of 66) | 93.9 % (31 of 33) | 84.4 % (27 of 32) | 100.0 % (1 of 1) |
Methyl | 87.5 % (119 of 136) | 89.7 % (61 of 68) | 85.3 % (58 of 68) |
1. HMG ACPI
SGLVPRGSHM TPQVNQVNLS EIKQVLKQQL AEALYTEESE IAEDQKFVDL GLDXIVGVEW TTTINQTYNL NLKATKLYDY PTLLELSGYI AQILSSQGTSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 291 K, pH 6.8, Details 1mM holo ACPI in 50mM Arg and 50mM Glu; pH= 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HMG_ACPI | [U-98% 15N] | 1 mM | |
2 | D2O | natural abundance | 5 % | |
3 | DSS | natural abundance | 0.15 mM | |
4 | Glutamate | natural abundance | 50 mM | |
5 | Arginine | natural abundance | 50 mM | |
6 | H2O | natural abundance | 95 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 291 K, pH 6.8, Details 1mM holo ACPI in 50mM Arg/Glu; pH= 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | HMG_ACPI | [U-13C] | 1 mM | |
8 | D2O | natural abundance | 5 % | |
9 | DSS | natural abundance | 0.15 mM | |
10 | Arginine | natural abundance | 50 mM | |
11 | Glutamate | natural abundance | 50 mM | |
12 | H2O | natural abundance | 95 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 291 K, pH 6.8, Details 1mM HMG-ACPI in 25mM NaPi, 50mM NaCl, pH=6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | HMG_ACPI | [U-13C] and [U-N15] | 1 mM | |
14 | D2O | natural abundance | 0.15 mM | |
15 | DSS | natural abundance | 5 % | |
16 | NaCL | natural abundance | 50 mM | |
17 | NaPi | natural abundance | 25 mM | |
18 | H2O | natural abundance | 95 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 291 K, pH 6.8, Details 1mM in 50mM Arg/Glu pH=6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | HMG_ACPI | [U-15N] and (13C) | 1 mM | |
20 | D2O | natural abundance | 5 % | |
21 | DSS | natural abundance | 0.15 mM | |
22 | Glutamate | natural abundance | 50 mM | |
23 | Arginine | natural abundance | 50 mM | |
24 | H2O | natural abundance | 95 % |