BMRB: 17937

Solid-state Chemical Shift Assignments for CAP-Gly Domain of Mammalian Dynactin

Authors

Yan, S.

Assembly

CAP-Gly

Entity

1. CAP-Gly (polymer, Thiol state: all free), 88 monomers, 9425.397 Da Detail

TEASARPLRV GSRVEVIGKG HRGTVAYVGA TLFATGKWVG VILDEAKGKN DGTVQGRKYF TCDEGHGIFV RQSQIQVFED GADTTSPE

Formula weight

9425.397 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.5 %, Completeness (bb): 98.2 % Detail

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Polymer type: polypeptide(L)

	Total	13C	15N
All	85.5 % (402 of 470)	83.9 % (317 of 378)	92.4 % (85 of 92)
Backbone	98.2 % (331 of 337)	98.0 % (246 of 251)	98.8 % (85 of 86)
Sidechain	69.7 % (145 of 208)	71.8 % (145 of 202)	0.0 % (0 of 6)
Aromatic	10.5 % (4 of 38)	10.8 % (4 of 37)	0.0 % (0 of 1)
Methyl	87.8 % (43 of 49)	87.8 % (43 of 49)

1. CAP-Gly

TEASARPLRV GSRVEVIGKG HRGTVAYVGA TLFATGKWVG VILDEAKGKN DGTVQGRKYF TCDEGHGIFV RQSQIQVFED GADTTSPE

Show sample condition

Sample

Solvent system Water, Pressure 1 atm, Temperature 256.3 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	CAP-Gly	[U-100% 13C; U-100% 15N]		14 (±0.5) mg

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LACS Plot; CA

Referencing offset: 0.29 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.29 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 2M02, Strand ID: A Detail

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Release date

2011-10-27

Citation

Solid-state and solution NMR studies of the CAP-Gly domain of mammalian dynactin and its interaction with microtubules
Sun, S., Siglin, A., Williams, J.C., Polenova, T.
J. Am. Chem. Soc. (2009), 131, 10113-10126, PubMed 19580321 , DOI 10.1021/ja902003u , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. CAP-Gly, : 1 : 4 : 9 : 117 entities Detail

Interaction partners 1. CAP-Gly, : 4 interactors Detail

Experiments performed 10 experiments Detail