BMRB: 17938

Solution Chemical Shifts Assignments of 1H, 13C, and 15N for CAP-Gly

Authors

Yan, S.

Assembly

CAP-Gly

Entity

1. CAP-Gly (polymer, Thiol state: all free), 88 monomers, 9425.397 Da Detail

TEASARPLRV GSRVEVIGKG HRGTVAYVGA TLFATGKWVG VILDEAKGKN DGTVQGRKYF TCDEGHGIFV RQSQIQVFED GADTTSPE

Formula weight

9425.397 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 65.5 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	65.5 % (633 of 967)	59.4 % (295 of 497)	66.7 % (252 of 378)	93.5 % (86 of 92)
Backbone	98.1 % (514 of 524)	97.3 % (182 of 187)	98.0 % (246 of 251)	100.0 % (86 of 86)
Sidechain	37.1 % (192 of 518)	36.5 % (113 of 310)	39.1 % (79 of 202)	0.0 % (0 of 6)
Aromatic	0.0 % (0 of 76)	0.0 % (0 of 38)	0.0 % (0 of 37)	0.0 % (0 of 1)
Methyl	21.4 % (21 of 98)	20.4 % (10 of 49)	22.4 % (11 of 49)

1. CAP-Gly

TEASARPLRV GSRVEVIGKG HRGTVAYVGA TLFATGKWVG VILDEAKGKN DGTVQGRKYF TCDEGHGIFV RQSQIQVFED GADTTSPE

Show sample condition

Sample

Solvent system Water, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	CAP-Gly	[U-100% 13C; U-100% 15N]		0.96 mM

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LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.3 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Release date

2011-10-27

Citation

Solid-state and solution NMR studies of the CAP-Gly domain of mammalian dynactin and its interaction with microtubules
Sun, S., Siglin, A., Williams, J.C., Polenova, T.
J. Am. Chem. Soc. (2009), 131, 10113-10126, PubMed 19580321 , DOI 10.1021/ja902003u , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. CAP-Gly, : 1 : 4 : 9 : 117 entities Detail

Interaction partners 1. CAP-Gly, : 4 interactors Detail

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail