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BMRB: 18188

6KG8

CohA2

Authors

Feng, Y., Cui, Z., Li, Y., Cui, Q.

Assembly

CohA2

Entity

1. CohA2 (polymer, Thiol state: all free), 155 monomers, 16017.71 Da Detail

GSASVKNETV KLSVGTVSGN PGDTVKVPVT ISQVSTPVGL ICMDISYDAS KFTVKDVLPN TDLVKDTDNY SFIVNTSTPG KISITFTDPT LANYPISVDG ILAYLDFIIN SNATAGDSAL TVDPATLIVA DENDKDIKDA ASNGKITVTG SAPTS

Formula weight

16017.71 Da

Source organism

Clostridium acetobutylicum

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 98.5 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.5 % (1631 of 1655)	98.9 % (826 of 835)	98.0 % (650 of 663)	98.7 % (155 of 157)
Backbone	98.5 % (898 of 912)	98.7 % (307 of 311)	98.2 % (447 of 455)	98.6 % (144 of 146)
Sidechain	98.8 % (877 of 888)	99.0 % (519 of 524)	98.3 % (347 of 353)	100.0 % (11 of 11)
Aromatic	94.4 % (68 of 72)	94.4 % (34 of 36)	94.4 % (34 of 36)
Methyl	97.7 % (213 of 218)	98.2 % (107 of 109)	97.2 % (106 of 109)

1. CohA2

GSASVKNETV KLSVGTVSGN PGDTVKVPVT ISQVSTPVGL ICMDISYDAS KFTVKDVLPN TDLVKDTDNY SFIVNTSTPG KISITFTDPT LANYPISVDG ILAYLDFIIN SNATAGDSAL TVDPATLIVA DENDKDIKDA ASNGKITVTG SAPTS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.8

#	Name	Isotope labeling	Concentration
1	CohA2	[U-100% 13C; U-100% 15N]	0.6 mM
2	sodium acetate	natural abundance	20 mM
3	potassium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	1 mM
5	sodium azide	natural abundance	0.01 % w/v
6	DSS	natural abundance	0.05 % w/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 1 Detail

Release date

2012-03-26

Citation

Resonance assignments of cohesin and dockerin domains from Clostridium acetobutylicum ATCC824
Cui, Z., Li, Y., Xiao, Y., Feng, Y., Cui, Q.
Biomol. NMR Assign. (2013), 7, 73-76, PubMed 22446850 , DOI 10.1007/s12104-012-9381-2 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18189 released on 2012-03-26
Title DocA

Related entities 1. CohA2, : 1 : 4 : 11 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC