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BMRB: 18428

N0N1 domains of Neisseria meningitidis Pilus assembly protein PilQ

Authors

Phelan, M.M., Berry, J., Derrick, J.P., Lian, L.

Assembly

PilQ

Entity

1. PilQ (polymer, Thiol state: not present), 237 monomers, 26141.51 Da Detail

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSFTGRKI SLDFQDVEIR TILQILAKES GMNIVASDSV NGKMTLSLKD VPWDQALDLV MQARNLDMRQ QGNIVNIAPR DELLAKDKAF LQAEKDIADL GALYSQNFQL KYKNVEEFRS ILRLDNADTT GNRNTLVSGR GSVLIDPATN TLIVTDTRSV IEKFRKLIDE LDVPAQQVMI EARIVEAADG FSRDLGVKFG ATGKKKL

Formula weight

26141.51 Da

Source organism

Neisseria meningitidis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.6 %, Completeness: 68.4 %, Completeness (bb): 89.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.4 % (1858 of 2716)	61.9 % (874 of 1413)	73.0 % (764 of 1046)	85.6 % (220 of 257)
Backbone	89.9 % (1269 of 1412)	88.1 % (430 of 488)	90.6 % (627 of 692)	91.4 % (212 of 232)
Sidechain	51.7 % (787 of 1522)	48.0 % (444 of 925)	58.6 % (335 of 572)	32.0 % (8 of 25)
Aromatic	20.6 % (28 of 136)	22.1 % (15 of 68)	17.9 % (12 of 67)	100.0 % (1 of 1)
Methyl	73.6 % (206 of 280)	73.6 % (103 of 140)	73.6 % (103 of 140)

1. PilQ N0N1

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	PilQ_N0N1	[U-98% 13C; U-98% 15N]	600 (±100.0) uM
2	sodium chloride	natural abundance	50 (±1.0) mM
3	potassium phosphate	natural abundance	50 (±1.0) mM
4	sodium azide	natural abundance	0.2 (±0.02) %
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Release date

2013-01-02

Citation

Structure and assembly of a trans-periplasmic channel for type IV pili in Neisseria meningitidis
Berry, J., Phelan, M.M., Collins, R.F., Adomavicius, T., Tnjum, T., Frye, S.A., Bird, L., Owens, R., Ford, R.C., Lian, L., Derrick, J.P.
PLoS Pathog. (2012), 8, e1002923-e1002923, PubMed 23028322 , DOI 10.1371/journal.ppat.1002923 , Abstract

Entries sharing articles BMRB: 2, EMDB: 1 entries Detail

  BMRB: 18419 released on 2013-01-02
    Title B2 domain of Neisseria meningitidis Pilus assembly protein PilQ
  BMRB: 18459 released on 2013-01-02
    Title N0 domain of Neisseria meningitidis Pilus assembly protein PilQ
  EMDB: 2105 released on 2012-10-03
    Title Single particle electron microscopy of PilQ dodecameric complexes from Neisseria meningitidis.

Related entities 1. PilQ, : 19 entities Detail

Interaction partners 1. PilQ, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 CBCACONH (H[N[co[{CA|ca[C]}]]])