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BMRB: 18458

5K5F

1H, 13C, 15N Chemical shift assignment of HIRAN domain of human HLTF

Authors

Bezsonova, I., Neculai, D., Arrowsmith, C.H., Dhe-Paganon, S.

Assembly

HIRAN domain

Entity

1. HIRAN domain (polymer, Thiol state: all free), 122 monomers, 13514.17 Da Detail

GSDEEVDSVL FGSLRGHVVG LRYYTGVVNN NEMVALQRDP NNPYDKNAIK VNNVNGNQVG HLKKELAGAL AYIMDNKLAQ IEGVVPFGAN NAFTMPLHMT FWGKEENRKA VSDQLKKHGF KL

Formula weight

13514.17 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.7 %, Completeness (bb): 99.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.7 % (1189 of 1420)	94.2 % (696 of 739)	68.2 % (371 of 544)	89.1 % (122 of 137)
Backbone	99.9 % (723 of 724)	99.6 % (251 of 252)	100.0 % (354 of 354)	100.0 % (118 of 118)
Sidechain	71.5 % (576 of 806)	91.4 % (445 of 487)	42.3 % (127 of 300)	21.1 % (4 of 19)
Aromatic	14.5 % (16 of 110)	23.6 % (13 of 55)	3.7 % (2 of 54)	100.0 % (1 of 1)
Methyl	64.4 % (85 of 132)	100.0 % (66 of 66)	28.8 % (19 of 66)

1. HLTF-HIRAN

GSDEEVDSVL FGSLRGHVVG LRYYTGVVNN NEMVALQRDP NNPYDKNAIK VNNVNGNQVG HLKKELAGAL AYIMDNKLAQ IEGVVPFGAN NAFTMPLHMT FWGKEENRKA VSDQLKKHGF KL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	HLTF-HIRAN	[U-100% 13C; U-100% 15N]	1-1.6 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Release date

2012-06-11

Citation

Solution NMR structure of the HLTF HIRAN domain: a conserved module in SWI2/SNF2 DNA damage tolerance proteins
Neculai, D., Bezsonova, I., Weigelt, J., Bountra, C., Edwards, A., Arrowsmith, C., Dhe-Paganon, S.
J. Biomol. NMR (2016), 66, 209-219, PubMed 27771863 , DOI 10.1007/s10858-016-0070-9 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 30098 released on 2016-06-05
    Title NMR structure of the HLTF HIRAN domain
  BMRB: 17085 released on 2012-05-13
    Title NMR structure of the HLTF HIRAN domain

Related entities 1. HIRAN domain, : 1 : 1 : 6 : 11 entities Detail

Interaction partners 1. HIRAN domain, : 30 interactors Detail

More details for 30 interactors identified by 14 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC