BMRB: 19025

Solid-state Chemical Shift Assignments for CAP-Gly Domain of Mammalian at 19.9 T

Authors

Yan, S., Hou, G., Schwieters, C.D., Ahmed, S., Williams, J.C., Polenova, T.

Assembly

CAP-Gly monomer

Entity

1. CAP-Gly monomer (polymer, Thiol state: all free), 89 monomers, 9512.475 Da Detail

STEASARPLR VGSRVEVIGK GHRGTVAYVG ATLFATGKWV GVILDEAKGK NDGTVQGRKY FTCDEGHGIF VRQSQIQVFE DGADTTSPE

Formula weight

9512.475 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 82.0 %, Completeness: 70.9 %, Completeness (bb): 80.6 % Detail

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Polymer type: polypeptide(L)

	Total	13C	15N
All	70.9 % (336 of 474)	69.3 % (264 of 381)	77.4 % (72 of 93)
Backbone	80.6 % (275 of 341)	79.9 % (203 of 254)	82.8 % (72 of 87)
Sidechain	57.9 % (121 of 209)	59.6 % (121 of 203)	0.0 % (0 of 6)
Aromatic	10.5 % (4 of 38)	10.8 % (4 of 37)	0.0 % (0 of 1)
Methyl	71.4 % (35 of 49)	71.4 % (35 of 49)

1. CAP-Gly

STEASARPLR VGSRVEVIGK GHRGTVAYVG ATLFATGKWV GVILDEAKGK NDGTVQGRKY FTCDEGHGIF VRQSQIQVFE DGADTTSPE

Show sample condition

Sample

Solvent system 100% H2O, Pressure 1 atm, Temperature 277 K, pH 6.0, Details Sample was precipitated by 30% PEG-3350.

#	Name	Isotope labeling	Type	Concentration
1	CAP-Gly	[U-13C; U-15N]		24.4 mg
2	H2O	natural abundance		100 %

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Chem. Shift Complete2

Sequence coverage: 83.1 %, Completeness: 70.9 %, Completeness (bb): 80.8 % Detail

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Polymer type: polypeptide(L)

	Total	13C	15N
All	70.9 % (672 of 948)	69.2 % (527 of 762)	78.0 % (145 of 186)
Backbone	80.8 % (551 of 682)	79.9 % (406 of 508)	83.3 % (145 of 174)
Sidechain	57.7 % (241 of 418)	59.4 % (241 of 406)	0.0 % (0 of 12)
Aromatic	10.5 % (8 of 76)	10.8 % (8 of 74)	0.0 % (0 of 2)
Methyl	71.4 % (70 of 98)	71.4 % (70 of 98)

1. CAP-Gly

STEASARPLR VGSRVEVIGK GHRGTVAYVG ATLFATGKWV GVILDEAKGK NDGTVQGRKY FTCDEGHGIF VRQSQIQVFE DGADTTSPE

Show sample condition

Sample

Solvent system 100% H2O, Pressure 1 atm, Temperature 277 K, pH 6.0, Details Sample was precipitated by 30% PEG-3350.

#	Name	Isotope labeling	Type	Concentration
1	CAP-Gly	[U-13C; U-15N]		24.4 mg
2	H2O	natural abundance		100 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Release date

2013-05-29

Citation

Three-dimensional structure of CAP-gly domain of mammalian dynactin determined by magic angle spinning NMR spectroscopy: conformational plasticity and interactions with end-binding protein EB1
Yan, S., Hou, G., Schwieters, C.D., Ahmed, S., Williams, J.C., Polenova, T.
J. Mol. Biol. (2013), 425, 4249-4266, PubMed 23648839 , DOI 10.1016/j.jmb.2013.04.027 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. CAP-Gly monomer, : 1 : 4 : 9 : 104 entities Detail

Interaction partners 1. CAP-Gly monomer, : 4 interactors Detail

Experiments performed 5 experiments Detail