BMRBj - BMREntryMaster

Found 1 Document (0.535 seconds)

BMRB: 19686

2MGZ

ASD-1 RRM domain bound to UGCAUGG RNA

Authors

Amrane, S., Mackereth, C.D.

Assembly

ASD-1+RNA

Entity

1. ASD-1 (polymer, Thiol state: not present), 99 monomers, 10883.98 Da Detail

GAMGPVAPND RSTSSSSTDG PRRLHVSNIP FKYREPDLTA MFEKVGPVVD VEIIFNERGS KGFGFVTMQN PDDADRARAE FNGTTIEGRR VEVNLATQR

2. UGCAUGG (polymer, Thiol state: not present), 7 monomers, 2300.351 Da Detail

UGCAUGG

Total weight

13184.332 Da

Max. entity weight

10883.98 Da

Source organism

Caenorhabditis elegans

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 30.4 %, Completeness (bb): 56.0 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	30.4 % (370 of 1217)	14.3 % (91 of 638)	40.3 % (191 of 474)	83.8 % (88 of 105)
Backbone	56.0 % (368 of 657)	36.8 % (89 of 242)	59.1 % (191 of 323)	95.7 % (88 of 92)
Sidechain	13.8 % (90 of 650)	0.5 % (2 of 396)	36.5 % (88 of 241)	0.0 % (0 of 13)
Aromatic	0.0 % (0 of 104)	0.0 % (0 of 52)	0.0 % (0 of 47)	0.0 % (0 of 5)
Methyl	7.6 % (7 of 92)	0.0 % (0 of 46)	15.2 % (7 of 46)

1. ASD-1

GAMGPVAPND RSTSSSSTDG PRRLHVSNIP FKYREPDLTA MFEKVGPVVD VEIIFNERGS KGFGFVTMQN PDDADRARAE FNGTTIEGRR VEVNLATQR

2. UGCAUGG

UGCAUGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	ASD-1	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	300 mM
4	D2O	[U-2H]	10 %
5	UGCAUGG	natural abundance	0.25 mM
6	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.41 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.41 ppm, Outliers: 1 Detail

Release date

2014-08-28

Citation

Backbone-independent nucleic acid binding by splicing factor SUP-12 reveals key aspects of molecular recognition
Amrane, S., Rebora, K., Zniber, I., Dupuy, D., Mackereth, C.D.
Nat. Commun. (2014), 5, 4595-4595, PubMed 25183497 , DOI 10.1038/ncomms5595 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 19680 released on 2014-08-28
    Title ASD-1 RRM domain
  BMRB: 19653 released on 2014-02-10
    Title RRM domain from C. elegans SUP-12

Related entities 1. ASD-1, : 1 : 153 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr19686_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr19686_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	359		98.0 (chain: 1, length: 99)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 99, Sequence coverage: 98.0%
- Total number of assignments
  - ¹³C chemical shifts: 185
  - ¹H chemical shifts: 87
  - ¹⁵N chemical shifts: 87
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	428	185	43.2
¹H chemical shifts	578	87	15.1
¹⁵N chemical shifts	110	87	79.1

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.535 seconds)

BMRB: 19686

Sample

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. ASD-1, : 1 : 153 entities Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	198	97	49.0
¹H chemical shifts	200	87	43.5
¹⁵N chemical shifts	92	87	94.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	230	88	38.3
¹H chemical shifts	378	0	0.0
¹⁵N chemical shifts	18	0	0.0