BMRB: 19905

Phosphorylated 4E-BP2

Authors

Bah, A., Forman-Kay, J.

Assembly

phosphorylated 4E-BP2 monomer

Entity

1. phosphorylated 4E-BP2 monomer (polymer, Thiol state: all free), 120 monomers, 12939.23 Da Detail

MSSSAGSGHQ PSQSRAIPTR TVAISDAAQL PHDYCTTPGG TLFSTTPGGT RIIYDRKFLL DRRNSPMAQT PPCHLPNIPG VTSPGTLIED SKVEVNNLNN LNNHDRKHAV GDDAQFEMDI

Formula weight

12939.23 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 46.1 %, Completeness (bb): 87.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	46.1 % (549 of 1192)	34.4 % (235 of 683)	61.7 % (314 of 509)
Backbone	87.1 % (513 of 589)	90.3 % (215 of 238)	84.9 % (298 of 351)
Sidechain	17.6 % (126 of 714)	4.0 % (18 of 445)	40.1 % (108 of 269)
Aromatic	3.0 % (2 of 66)	3.0 % (1 of 33)	3.0 % (1 of 33)
Methyl	16.9 % (20 of 118)	8.5 % (5 of 59)	25.4 % (15 of 59)

1. Phosphorylated 4E-BP2

MSSSAGSGHQ PSQSRAIPTR TVAISDAAQL PHDYCTTPGG TLFSTTPGGT RIIYDRKFLL DRRNSPMAQT PPCHLPNIPG VTSPGTLIED SKVEVNNLNN LNNHDRKHAV GDDAQFEMDI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	Phosphorylated 4E-BP2	[U-99% 13C; U-99% 15N]		1 mM
2	DTT	natural abundance		2 mM
3	sodium chloride	natural abundance		100 mM
4	sodium phosphate	natural abundance		30 mM
5	EDTA	natural abundance		1 mM
6	Benzamidine	natural abundance		1 mM

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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Release date

2014-12-08

Citation

Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch
Bah, A., Vernon, R., Siddiqui, Z., Krzeminski, M., Muhandiram, R., Zhao, C., Sonenberg, N., Kay, L., Forman-Kay, J.
Nature (2015), 519, 106-109, PubMed 25533957 , DOI 10.1038/nature13999 , Abstract

Related entities 1. phosphorylated 4E-BP2 monomer, : 2 : 18 entities Detail

Interaction partners 1. phosphorylated 4E-BP2 monomer, : 3 interactors Detail

Experiments performed 6 experiments Detail