BMRBj - BMREntryMaster

Found 1 Document (0.558 seconds)

BMRB: 25093

1MVF

full-length EcMazE

Authors

Zangger, K., van Nuland, N.

Assembly

EcMazE homodimer

Entity

1. EcMazE homodimer (polymer, Thiol state: not present), 82 monomers, 9355.612 × 2 Da Detail

MIHSSVKRWG NSPAVRIPAT LMQALNLNID DEVKIDLVDG KLIIEPVRKE PVFTLAELVN DITPENLHEN IDWGEPKDKE VW

Total weight

18711.225 Da

Max. entity weight

9355.612 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 82.6 %, Completeness (bb): 94.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.6 % (809 of 980)	79.3 % (405 of 511)	85.4 % (327 of 383)	89.5 % (77 of 86)
Backbone	94.0 % (451 of 480)	89.4 % (144 of 161)	96.3 % (234 of 243)	96.1 % (73 of 76)
Sidechain	75.0 % (434 of 579)	74.6 % (261 of 350)	77.2 % (169 of 219)	40.0 % (4 of 10)
Aromatic	11.1 % (6 of 54)	18.5 % (5 of 27)	0.0 % (0 of 24)	33.3 % (1 of 3)
Methyl	86.4 % (95 of 110)	89.1 % (49 of 55)	83.6 % (46 of 55)

1. full EcMazE free

MIHSSVKRWG NSPAVRIPAT LMQALNLNID DEVKIDLVDG KLIIEPVRKE PVFTLAELVN DITPENLHEN IDWGEPKDKE VW

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE free	[U-99% 13C; U-99% 15N]	1 mM
2	H20	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
4	full EcMazE free	[U-99% 13C; U-99% 15N]		1 mM
5	D2O	natural abundance		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.83 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.83 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.47 ppm, Outliers: 1 Detail

Release date

2015-02-01

Citation

Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding
Zorzini, V., Buts, L., Schrank, E., Sterckx, Y.G.J., Respondek, M., Engelberg-Kulka, H., Loris, R., Zangger, K., van Nuland, N.A.J.
Nucleic Acids Res. (2015), 43, 1241-1256, PubMed 25564525 , DOI 10.1093/nar/gku1352 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 25086 released on 2015-02-01
    Title truncated EcMazE
  BMRB: 25092 released on 2015-02-01
    Title truncated EcMazE-DNA complex
  BMRB: 25094 released on 2015-02-01
    Title full-length EcMazE-DNA complex

Related entities 1. EcMazE homodimer, : 1 : 3 : 5 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE free	[U-99% 13C; U-99% 15N]	1 mM
2	H20	natural abundance	90 %
3	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE free	[U-99% 13C; U-99% 15N]	1 mM
2	H20	natural abundance	90 %
3	D2O	natural abundance	10 %

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE free	[U-99% 13C; U-99% 15N]	1 mM
2	H20	natural abundance	90 %
3	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE free	[U-99% 13C; U-99% 15N]	1 mM
2	H20	natural abundance	90 %
3	D2O	natural abundance	10 %

5 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE free	[U-99% 13C; U-99% 15N]	1 mM
2	H20	natural abundance	90 %
3	D2O	natural abundance	10 %

6 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE free	[U-99% 13C; U-99% 15N]	1 mM
2	H20	natural abundance	90 %
3	D2O	natural abundance	10 %

7 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
4	full EcMazE free	[U-99% 13C; U-99% 15N]		1 mM
5	D2O	natural abundance		100 %

8 2D 1H-1H TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE free	[U-99% 13C; U-99% 15N]	1 mM
2	H20	natural abundance	90 %
3	D2O	natural abundance	10 %

9 2D 1H-1H NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	full EcMazE free	[U-99% 13C; U-99% 15N]	1 mM
2	H20	natural abundance	90 %
3	D2O	natural abundance	10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr25093_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr25093_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	913		98.8 (chain: 1, length: 82)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 82, Sequence coverage: 98.8%
- Total number of assignments
  - ¹H chemical shifts: 511
  - ¹³C chemical shifts: 323
  - ¹⁵N chemical shifts: 79
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
18	PRO	N	119.897

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	511	411	80.4
¹³C chemical shifts	383	323	84.3
¹⁵N chemical shifts	89	78	87.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	161	149	92.5
¹³C chemical shifts	164	158	96.3
¹⁵N chemical shifts	76	72	94.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	350	262	74.9
¹³C chemical shifts	219	165	75.3
¹⁵N chemical shifts	13	6	46.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	57	50	87.7
¹³C chemical shifts	57	45	78.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	5	18.5
¹³C chemical shifts	24	0	0.0
¹⁵N chemical shifts	3	1	33.3

Keywords MazEF, intrinsic disorder, homodimer, Toxin-Antitoxin

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Found 1 Document (0.558 seconds)

BMRB: 25093

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. EcMazE homodimer, : 1 : 3 : 5 entities Detail

Experiments performed 9 experiments Detail

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Chemical shift validation 3 contents Detail