1H, 13C and 15N chemical shift assignments for PARP-1 F1F2 domains (at 200mM NaCl)
MAESSDKLYR VEYAKSGRAS CKKCSESIPK DSLRMAIMVQ SPMFDGKVPH WYHFSCFWKV GHSIRHPDVE VDGFSELRWD DQQKVKKTAE AGGVTGKGQD GIGSKAEKTL GDFAAEYAKS NRSTCKGCME KIEKGQVRLS KKMVDPEKPQ LGMIDRWYHP GCFVKNREEL GFRPEYSASQ LKGFSLLATE DKEALKKQLP GVKSEGKRKG DEVD
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 26.6 % (413 of 1550) | 16.7 % (222 of 1331) | 87.2 % (191 of 219) |
Backbone | 60.4 % (389 of 644) | 45.1 % (198 of 439) | 93.2 % (191 of 205) |
Sidechain | 2.8 % (25 of 906) | 2.8 % (25 of 892) | 0.0 % (0 of 14) |
Aromatic | 0.0 % (0 of 102) | 0.0 % (0 of 98) | 0.0 % (0 of 4) |
Methyl | 2.5 % (2 of 80) | 2.5 % (2 of 80) |
1. F1F2
MAESSDKLYR VEYAKSGRAS CKKCSESIPK DSLRMAIMVQ SPMFDGKVPH WYHFSCFWKV GHSIRHPDVE VDGFSELRWD DQQKVKKTAE AGGVTGKGQD GIGSKAEKTL GDFAAEYAKS NRSTCKGCME KIEKGQVRLS KKMVDPEKPQ LGMIDRWYHP GCFVKNREEL GFRPEYSASQ LKGFSLLATE DKEALKKQLP GVKSEGKRKG DEVDSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details Uniform [2H,15N,13C]
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PARP-1 1-214 | [U-15N; U-13C; U-2H] | 0.2 mM | |
2 | TRIS | [U-2H] | 50 mM | |
3 | DTT | [U-2H] | 1 mM | |
4 | ZnSO4 | natural abundance | 0.1 uM | |
5 | sodium chloride | natural abundance | 200 mM | |
6 | H2O | natural abundance | solvent | 95 % |
7 | D2O | [U-2H] | solvent | 5 % |