Tom1 negatively modulates binding of Tollip to phosphatidylinositol 3-phosphate via a coupled folding and binding mechanism
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 38.4 % (247 of 643) | 16.5 % (55 of 334) | 59.4 % (145 of 244) | 72.3 % (47 of 65) |
Backbone | 69.7 % (237 of 340) | 40.0 % (48 of 120) | 85.5 % (142 of 166) | 87.0 % (47 of 54) |
Sidechain | 15.0 % (53 of 353) | 3.3 % (7 of 214) | 35.9 % (46 of 128) | 0.0 % (0 of 11) |
Aromatic | 0.0 % (0 of 26) | 0.0 % (0 of 13) | 0.0 % (0 of 13) | |
Methyl | 15.7 % (11 of 70) | 8.6 % (3 of 35) | 22.9 % (8 of 35) |
1. TBD
GPLGSMATTV STQRGPVYIG ELPQDFLRIT PTQQQRQVQL DAQAAQQLQY GGAVGTVGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TBD | [U-99% 13C; U-99% 15N] | 1.0 mM | |
2 | potassium chloride | natural abundance | 50 mM | |
3 | sodium azide | natural abundance | 1 mM | |
4 | TRIS | [U-2H] | 20 mM | |
5 | DSS | natural abundance | 50 uM | |
6 | DTT | [U-2H] | 1 mM |