BMRB: 26573

Tom1 negatively modulates binding of Tollip to phosphatidylinositol 3-phosphate via a coupled folding and binding mechanism

Authors

Xiao, S., Capelluto, D.

Assembly

TBD

Entity

1. TBD (polymer, Thiol state: not present), 58 monomers, 6128.791 Da Detail

GPLGSMATTV STQRGPVYIG ELPQDFLRIT PTQQQRQVQL DAQAAQQLQY GGAVGTVG

Formula weight

6128.791 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.7 %, Completeness: 38.4 %, Completeness (bb): 69.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	38.4 % (247 of 643)	16.5 % (55 of 334)	59.4 % (145 of 244)	72.3 % (47 of 65)
Backbone	69.7 % (237 of 340)	40.0 % (48 of 120)	85.5 % (142 of 166)	87.0 % (47 of 54)
Sidechain	15.0 % (53 of 353)	3.3 % (7 of 214)	35.9 % (46 of 128)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 26)	0.0 % (0 of 13)	0.0 % (0 of 13)
Methyl	15.7 % (11 of 70)	8.6 % (3 of 35)	22.9 % (8 of 35)

1. TBD

GPLGSMATTV STQRGPVYIG ELPQDFLRIT PTQQQRQVQL DAQAAQQLQY GGAVGTVG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	TBD	[U-99% 13C; U-99% 15N]		1.0 mM
2	potassium chloride	natural abundance		50 mM
3	sodium azide	natural abundance		1 mM
4	TRIS	[U-2H]		20 mM
5	DSS	natural abundance		50 uM
6	DTT	[U-2H]		1 mM

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LACS Plot; CA

Referencing offset: 2.61 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.61 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.92 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Release date

2015-12-21

Citation

Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism
Xiao, S., Brannon, M.K., Zhao, X., Fread, K.I., Ellena, J.F., Bushweller, J.H., Finkielstein, C.V., Armstrong, G.S., Capelluto, D.G.S.
Structure (2015), 23, 1910-1920, PubMed 26320582 , DOI 10.1016/j.str.2015.07.017 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. TBD, : 2 : 15 entities Detail

Interaction partners 1. TBD, : 43 interactors Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail